BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10336

Title: Solution structure of the DUF1000 domain of a thioredoxin-like protein 1   PubMed: 20455272

Authors: Goroncy, A.; Kigawa, T.; Koshiba, S.; Kobayashi, N.; Tochio, N.; Inoue, M.; Yokoyama, S.

Citation: Goroncy, Alexander; Koshiba, Seizo; Tochio, Naoya; Tomizawa, Tadashi; Inoue, Makoto; Tanaka, Akiko; Sugano, Sumio; Kigawa, Takanori; Yokoyama, Shigeyuki. "Solution structure of the C-terminal DUF1000 domain of the human thioredoxin-like 1 protein"  Proteins 78, 2176-2180 (2010).

Assembly members:
DUF1000 domain, polymer, 171 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: Cell free synthesis

Entity Sequences (FASTA):
DUF1000 domain: GSSGSSGGYMDLMPFINKAG CECLNESDEHGFDNCLRKDT TFLESDCDEQLLITVAFNQP VKLYSMKFQGPDNGQGPKYV KIFINLPRSMDFEEAERSEP TQALELTEDDIKEDGIVPLR YVKFQNVNSVTIFVQSNQGE EETTRISYFTFIGTPVQATN MNDFKSGPSSG

Data sets:
Data typeCount
13C chemical shifts752
15N chemical shifts183
1H chemical shifts1172

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DUF1000 domain1

Entities:

Entity 1, DUF1000 domain 171 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLYTYRMET
2   ASPLEUMETPROPHEILEASNLYSALAGLY
3   CYSGLUCYSLEUASNGLUSERASPGLUHIS
4   GLYPHEASPASNCYSLEUARGLYSASPTHR
5   THRPHELEUGLUSERASPCYSASPGLUGLN
6   LEULEUILETHRVALALAPHEASNGLNPRO
7   VALLYSLEUTYRSERMETLYSPHEGLNGLY
8   PROASPASNGLYGLNGLYPROLYSTYRVAL
9   LYSILEPHEILEASNLEUPROARGSERMET
10   ASPPHEGLUGLUALAGLUARGSERGLUPRO
11   THRGLNALALEUGLULEUTHRGLUASPASP
12   ILELYSGLUASPGLYILEVALPROLEUARG
13   TYRVALLYSPHEGLNASNVALASNSERVAL
14   THRILEPHEVALGLNSERASNGLNGLYGLU
15   GLUGLUTHRTHRARGILESERTYRPHETHR
16   PHEILEGLYTHRPROVALGLNALATHRASN
17   METASNASPPHELYSSERGLYPROSERSER
18   GLY

Samples:

sample_1: DUF1000 domain, [U-13C; U-15N], 1.02 mM; Tris-d11-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1
CBCACONHsample_1isotropiccondition_1
CCCONNHsample_1isotropiccondition_1
CCH TOCSYsample_1isotropiccondition_1
HBHACONHsample_1isotropiccondition_1
HCCCONNHsample_1isotropiccondition_1
HCCH COSYsample_1isotropiccondition_1
HCCH TOCSYsample_1isotropiccondition_1
3D HNCAsample_1isotropiccondition_1
3D HNCACBsample_1isotropiccondition_1
3D HNCACOsample_1isotropiccondition_1
3D HNCOsample_1isotropiccondition_1
3D HNCOCAsample_1isotropiccondition_1
C13 HSQCsample_1isotropiccondition_1
N15 HSQCsample_1isotropiccondition_1
3D 1H-13C NOESY-HSQCsample_1isotropiccondition_1
3D 1H-15N NOESY-HSQCsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker Biospin - collection

NMRPipe v20020425, FRANK DELAGLIO - processing

NMRView v5.0.4, BRUCE A. JOHNSON - data analysis

Kujira v0.896, NAOHIRO KOBAYASHI - data analysis

CYANA v2, PETER GUENTERT - refinement

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
DBJ BAC26626 BAD92500 BAE25774 BAE29469 BAE29934
GB AAC05830 AAC39599 AAC39898 AAC40183 AAF66676
REF NP_001071354 NP_001131150 NP_001231205 NP_001248637 NP_004777
SP O43396 Q8CDN6 Q920J4
TPG DAA15792