BMRB Entry 10327

Title:
Solution structure of the filamin domain from human tripartite motif protein 45
Deposition date:
2009-04-02
Original release date:
2010-04-07
Authors:
Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the filamin domain from human tripartite motif protein 45"  .

Assembly members:

Assembly members:
Filamin domain, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P060227-22

Data sets:
Data typeCount
13C chemical shifts488
15N chemical shifts113
1H chemical shifts765

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Filamin domain1

Entities:

Entity 1, Filamin domain 113 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUVALASP
2   PROALALYSCYSVALLEUGLNGLYGLUASP
3   LEUHISARGALAARGGLULYSGLNTHRALA
4   SERPHETHRLEULEUCYSLYSASPALAALA
5   GLYGLUILEMETGLYARGGLYGLYASPASN
6   VALGLNVALALAVALVALPROLYSASPLYS
7   LYSASPSERPROVALARGTHRMETVALGLN
8   ASPASNLYSASPGLYTHRTYRTYRILESER
9   TYRTHRPROLYSGLUPROGLYVALTYRTHR
10   VALTRPVALCYSILELYSGLUGLNHISVAL
11   GLNGLYSERPROPHETHRVALTHRVALARG
12   ARGLYSHIS

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 1.15 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB14484 BAD96621
GB AAH34943 AAT76864 ABZ92546 EAW56668
REF NP_001139107 XP_003806334 XP_004026476 XP_008950245

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks