BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10291

Title: The solution structure of the homeobox domain of human homeobox protein TGIF2LX

Authors: Tochio, N.; Ohnishi, S.; Sasagawa, A.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Tochio, N.; Ohnishi, S.; Sasagawa, A.; Saito, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the homeobox domain of human homeobox protein TGIF2LX"  . ., .-..

Assembly members:
homeobox domain, polymer, 83 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
homeobox domain: GSSGSSGKKRKGNLPAESVK ILRDWMYKHRFKAYPSEEEK QMLSEKTNLSLLQISNWFIN ARRRILPDMLQQRRNDPSGP SSG

Data sets:
Data typeCount
13C chemical shifts318
15N chemical shifts68
1H chemical shifts498

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1homeobox domain1

Entities:

Entity 1, homeobox domain 83 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSLYSARG
2   LYSGLYASNLEUPROALAGLUSERVALLYS
3   ILELEUARGASPTRPMETTYRLYSHISARG
4   PHELYSALATYRPROSERGLUGLUGLULYS
5   GLNMETLEUSERGLULYSTHRASNLEUSER
6   LEULEUGLNILESERASNTRPPHEILEASN
7   ALAARGARGARGILELEUPROASPMETLEU
8   GLNGLNARGARGASNASPPROSERGLYPRO
9   SERSERGLY

Samples:

sample_1: homeobox domain, [U-13C; U-15N], 1.1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.955, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAF85298 BAG74238 BAG74239
EMBL CAC87895 CAC87896 CAD20750 CAD20751
GB AAI01145 AAI01146 AAI17399 AAI17401 AAI28605
REF NP_001009067 NP_001266647 NP_620410 NP_631960 XP_003810418
SP A1YGI6 Q8IUE0 Q8IUE1 Q8MIB7 Q8MIE9