BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10094

Title: Solution structure of the third CUT domain of human Homeobox protein Cux-2

Authors: Nameki, N.; Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.

Citation: Nameki, N.; Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the third CUT domain of human Homeobox protein Cux-2"  . ., .-..

Assembly members:
CUT domain, polymer, 111 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
CUT domain: GSSGSSGYSGSQAPGGIQEI VAMSPELDTYSITKRVKEVL TDNNLGQRLFGESILGLTQG SVSDLLSRPKPWHKLSLKGR EPFVRMQLWLNDPHNVEKLR DMKKLSGPSSG

Data sets:
Data typeCount
13C chemical shifts462
15N chemical shifts103
1H chemical shifts734

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Homeobox protein Cux-21

Entities:

Entity 1, Homeobox protein Cux-2 111 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTYRSERGLY
2   SERGLNALAPROGLYGLYILEGLNGLUILE
3   VALALAMETSERPROGLULEUASPTHRTYR
4   SERILETHRLYSARGVALLYSGLUVALLEU
5   THRASPASNASNLEUGLYGLNARGLEUPHE
6   GLYGLUSERILELEUGLYLEUTHRGLNGLY
7   SERVALSERASPLEULEUSERARGPROLYS
8   PROTRPHISLYSLEUSERLEULYSGLYARG
9   GLUPROPHEVALARGMETGLNLEUTRPLEU
10   ASNASPPROHISASNVALGLULYSLEUARG
11   ASPMETLYSLYSLEUSERGLYPROSERSER
12   GLY

Samples:

sample_1: CUT domain, [U-13C; U-15N], 1.1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.901, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - structure solution

OPALp, Billeter, M., Guntert, P., Koradi, R. - refinement

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
REF XP_002823799 XP_006109587 XP_009684359 XP_011887772