BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10083

Title: PDZ domain of human RIM2B

Authors: Nagashima, T.; Hayashi, F.; Yokoyama, S.

Citation: Nagashima, T.; Hayashi, F.; Yokoyama, S.. "PDZ domain of human RIM2B"  . ., .-..

Assembly members:
PDZ domain, polymer, 131 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
PDZ domain: GSSGSSGHSHSDKHPVTWQP SKDGDRLIGRILLNKRLKDG SVPRDSGAMLGLKVVGGKMT ESGRLCAFITKVKKGSLADT VGHLRPGDEVLEWNGRLLQG ATFEEVYNIILESKPEPQVE LVVSRSGPSSG

Data sets:
Data typeCount
13C chemical shifts561
15N chemical shifts136
1H chemical shifts899

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Regulating synaptic membrane exocytosis protein 21

Entities:

Entity 1, Regulating synaptic membrane exocytosis protein 2 131 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYHISSERHIS
2   SERASPLYSHISPROVALTHRTRPGLNPRO
3   SERLYSASPGLYASPARGLEUILEGLYARG
4   ILELEULEUASNLYSARGLEULYSASPGLY
5   SERVALPROARGASPSERGLYALAMETLEU
6   GLYLEULYSVALVALGLYGLYLYSMETTHR
7   GLUSERGLYARGLEUCYSALAPHEILETHR
8   LYSVALLYSLYSGLYSERLEUALAASPTHR
9   VALGLYHISLEUARGPROGLYASPGLUVAL
10   LEUGLUTRPASNGLYARGLEULEUGLNGLY
11   ALATHRPHEGLUGLUVALTYRASNILEILE
12   LEUGLUSERLYSPROGLUPROGLNVALGLU
13   LEUVALVALSERARGSERGLYPROSERSER
14   GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.17 mM; PiNa 20 mM; NaCl 100 mM; DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8992, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA34471 BAB18975 BAC98016 BAG10377 BAG54403
EMBL CAH18227
GB AAC19157 AAF81644 AAF81645 AAF81646 AAF81647
REF NP_001093587 NP_001243311 NP_001243312 NP_001269810 NP_055492
SP Q9EQZ7 Q9JIS1 Q9UQ26