BMRB Entry 10077

Title:
Solution structure of the RNA binding domain of eukaryotic initiation factor 4B
Deposition date:
2006-12-25
Original release date:
2008-08-14
Authors:
Suzuki, S.; Muto, Y.; Nagata, T.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.
Citation:

Citation: Suzuki, S.; Muto, Y.; Nagata, T.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of the RNA binding domain of eukaryotic initiation factor 4B"  .

Assembly members:

Assembly members:
RNA recognition motif, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P040301-18

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts415
15N chemical shifts93
1H chemical shifts647

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Eukaryotic translation initiation factor 4B1

Entities:

Entity 1, Eukaryotic translation initiation factor 4B 104 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERARGLEU
2   PROLYSSERPROPROTYRTHRALAPHELEU
3   GLYASNLEUPROTYRASPVALTHRGLUGLU
4   SERILELYSGLUPHEPHEARGGLYLEUASN
5   ILESERALAVALARGLEUPROARGGLUPRO
6   SERASNPROGLUARGLEULYSGLYPHEGLY
7   TYRALAGLUPHEGLUASPLEUASPSERLEU
8   LEUSERALALEUSERLEUASNGLUGLUSER
9   LEUGLYASNLYSARGILEARGVALASPVAL
10   ALAASPGLNALAGLNASPLYSASPSERGLY
11   PROSERSERGLY

Samples:

sample_1: RNA recognition motif, [U-13C; U-15N], 1.0 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v2.3, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.901, Kobayashi, N. - data analysis

Olivia v1.9.12, Yokochi, M. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB82380 BAD96248 BAG11107 BAG57157 BAG61335
EMBL CAA39265 CAG33239 CAH92323
GB AAH73139 AAH73154 AAH98437 AAI05158 AIC54329
REF NP_001030200 NP_001182737 NP_001287750 NP_001408 XP_001115324
SP P23588
TPG DAA30015
AlphaFold P23588

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks