BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 10043

Title: Solution Structure of the N-terminal Domain of Mouse Putative Signal Recoginition Particle 54 (SRP54)

Authors: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the N-terminal Domain of Mouse Putative Signal Recoginition Particle 54 (SRP54)"  . ., .-..

Assembly members:
N-terminal domain, polymer, 99 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
N-terminal domain: GSSGSSGADLGRKITSALRS LSNATIINEEVLNAMLKEVC TALLEADVNIKLVKQLRENV KSAIDLEEMASGLNKRKMIQ HAVFKELVKVKVYSGPSSG

Data sets:
Data typeCount
13C chemical shifts407
15N chemical shifts103
1H chemical shifts683

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Signal Recoginition Particle 541

Entities:

Entity 1, Signal Recoginition Particle 54 99 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYALAASPLEU
2   GLYARGLYSILETHRSERALALEUARGSER
3   LEUSERASNALATHRILEILEASNGLUGLU
4   VALLEUASNALAMETLEULYSGLUVALCYS
5   THRALALEULEUGLUALAASPVALASNILE
6   LYSLEUVALLYSGLNLEUARGGLUASNVAL
7   LYSSERALAILEASPLEUGLUGLUMETALA
8   SERGLYLEUASNLYSARGLYSMETILEGLN
9   HISALAVALPHELYSGLULEUVALLYSVAL
10   LYSVALTYRSERGLYPROSERSERGLY

Samples:

sample_1: Protein, [U-13C, U-15N], 1.04 mM; Phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.897, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
GB EAW65895 ELK11325 ELW63179 EPQ06973 KFO29291
REF XP_004376574 XP_005989239 XP_010211978 XP_012513641 XP_012867695