BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10014

Title: Assignments of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus   PubMed: 16838833

Authors: Okada, Kiyoshi; Takahashi, Mari; Sakamoto, Taiichi; Nakamura, Kouji; Kanai, Akio; Kawai, Gota

Citation: Okada, Kiyoshi; Takahashi, Mari; Sakamoto, Taiichi; Kawai, Gota; Nakamura, Kouji; Kanai, Akio. "Solution structure of a GAAG tetraloop in helix 6 of SRP RNA from Pyrococcus furiosus"  Nucleos. Nucleot. Nucl. 25, 383-395 (2006).

Assembly members:
pfHE6-12, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 186497   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Pyrococcus Pyrococcus furiosus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
pfHE6-12: GGCUGAAGGGCC

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts64

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1helix6 in SRP RNA1

Entities:

Entity 1, helix6 in SRP RNA 12 residues - Formula weight is not available

1   GGCUGAAGGG
2   CC

Samples:

he6_1: pfHE6-12 2.0 ± 0.1 mM; sodium chloride 50 ± 0.1 mM; phosphate buffer Na 20 ± 0.1 mM

condition_1: pH: 6.5; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
NOESYhe6_1not availablecondition_1
TOCSYhe6_1not availablecondition_1
1H-13C HSQChe6_1not availablecondition_1
DQF-COSYhe6_1not availablecondition_1

Software:

xwinnmr v2.6 -

FELIX v97.0 -

DISCOVER v97.0 -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links: