BMRB Entry 7352

Title:
SOLUTION STRUCTURE OF THE ZN COMPLEX OF EIAV NCP11(22-58) PEPTIDE, INCLUDING TWO CCHC ZN-BINDING MOTIFS.
Deposition date:
2006-12-01
Original release date:
2007-06-06
Authors:
Amodeo, P.; Castiglione-Morelli, M.; Ostuni, A.; Bavoso, A.
Citation:

Citation: Amodeo, P.; Castiglione-Morelli, M.; Ostuni, A.; Battistuzzi, G.; Bavoso, A.. "Structural Features in Eiav Ncp11: A Lentivirus Nucleocapsid Protein with a Short Linker"  Biochemistry 45, 5517-5526 (2006).
PubMed: 16634633

Assembly members:

Assembly members:
GAG_POLYPROTEIN_-_NUCLEOCAPSID_PROTEIN_P11, polymer, 37 residues, 65.409 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: HIV-2   Taxonomy ID: 11709   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus HIV-2

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GAG_POLYPROTEIN_-_NUCLEOCAPSID_PROTEIN_P11: QTCYNCGKPGHLSSQCRAPK VCFKCKQPGHFSKQCRS

Data sets:
Data typeCount
1H chemical shifts255

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GAG POLYPROTEIN1
2ZINC ION, 12
3ZINC ION, 22

Entities:

Entity 1, GAG POLYPROTEIN 37 residues - 65.409 Da.

1   GLNTHRCYSTYRASNCYSGLYLYSPROGLY
2   HISLEUSERSERGLNCYSARGALAPROLYS
3   VALCYSPHELYSCYSLYSGLNPROGLYHIS
4   PHESERLYSGLNCYSARGSER

Entity 2, ZINC ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample: NCp8 2.5 mM; H2O 90%; D2O 10%; TCEP.HCl 37.5 mM; zinc chloride 7.5 mM

sample_conditions_1: ionic strength: 210 mM; pH: 5.8; pressure: 1.0 atm; temperature: 293.0 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsampleisotropicsample_conditions_1
TOCSYsampleisotropicsample_conditions_1

Software:

AMBER, CASE ET AL - refinement, structure solution

Molmol v2K.2 - data analysis

VNMR v6.1C - processing

NMR spectrometers:

  • VARIAN UNITYINOVA 500 MHz

Related Database Links:

PDB
DBJ BAB12103 BAB12109
GB AAA43003 AAA43011 AAA43013 AAB59861 AAC03760
REF NP_056901
SP P69730 P69731 P69732
AlphaFold P69731 P69730 P69732