BMRB Entry 7341

Title:
CYCLIC MRIA: AN EXCEPTIONALLY STABLE AND POTENT CYCLIC CONOTOXIN WITH A NOVEL TOPOLOGICAL FOLD THAT TARGETS THE NOREPINEPHRINE TRANSPORTER.
Deposition date:
2006-11-30
Original release date:
2007-10-16
Authors:
Lovelace, E.; Armishaw, C.; Colgrave, M.; Wahlstrom, M.; Alewood, P.; Daly, N.; Craik, D.
Citation:

Citation: Lovelace, E.; Armishaw, C.; Colgrave, M.; Wahlstrom, M.; Alewood, P.; Daly, N.; Craik, D.. "Cyclic Mria: A Stable and Potent Cyclic Conotoxin with a Novel Topological Fold that Targets the Norepinephrine Transporter"  J. Med. Chem. 49, 6561-6568 (2006).
PubMed: 17064074

Assembly members:

Assembly members:
CYCLIC_MRIA, polymer, 15 residues, 131.130 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CYCLIC_MRIA: NGVCCGYKLCHXCAG

Data sets:
Data typeCount
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CYCLIC MRIA1

Entities:

Entity 1, CYCLIC MRIA 15 residues - 131.130 Da.

1   ASNGLYVALCYSCYSGLYTYRLYSLEUCYS
2   HISHYPCYSALAGLY

Samples:

sample: CYCLIC_MRIA 1 mM

sample_conditions_1: pH: 3.5; temperature: 280.0 K

Experiments:

NameSampleSample stateSample conditions
TOCSYsampleisotropicsample_conditions_1
NOESYsampleisotropicsample_conditions_1
COSYsampleisotropicsample_conditions_1
ECOSYsampleisotropicsample_conditions_1

Software:

CNS, BRUNGER,ADAMS,CLORE,DELANO,GROS, GROSSE-KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,READ,RICE,SIMONSON,WARREN - refinement, structure solution

NMR spectrometers:

  • BRUKER OTHER 600 MHz