BMRB Entry 7243

Title:
beta-microseminoprotein
Deposition date:
2006-07-25
Original release date:
2006-10-20
Authors:
Ghasriani, Houman; Teilum, Kaare; Johnsson, Ylva; Fernlund, Per; Drakenberg, Torbjorn
Citation:

Citation: Ghasriani, Houman; Teilum, Kaare; Johnsson, Ylva; Fernlund, Per; Drakenberg, Torbjorn. "Solution structures of human and porcine beta-microseminoprotein"  J. Mol. Biol. 362, 502-515 (2006).
PubMed: 16930619

Assembly members:

Assembly members:
beta-microseminoprotein, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Porcine   Taxonomy ID: 9823   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: sus scorfa

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts322
15N chemical shifts98
1H chemical shifts581

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pMSP1

Entities:

Entity 1, pMSP 94 residues - Formula weight is not available

1   GLYGLYGLYGLNCYSTYRPHEILEPROASN
2   GLNSERLEULYSPROASNGLUCYSGLNASP
3   LEULYSGLYVALSERHISPROLEUASNSER
4   VALTRPLYSTHRLYSASPCYSGLUGLUCYS
5   THRCYSGLYGLNASPALAILESERCYSCYS
6   ASNTHRALAALAILEPROTHRGLYTYRASP
7   THRASNLYSCYSGLNLYSILELEUASNLYS
8   LYSTHRCYSTHRTYRTHRVALVALGLULYS
9   LYSASPPROGLYLYSTHRCYSASPVALTHR
10   GLYTRPVALLEU

Samples:

sample_1: beta-microseminoprotein 4.0 ± 0.2 mM; H2O 90%; D2O 10%

sample_2: beta-microseminoprotein, [U-15N], 3.4 – 4.2 mM; H2O 90%; D2O 10%

sample_3: beta-microseminoprotein, [U-13C; U-15N], 3.4 – 4.2 mM; H2O 90%; D2O 10%

conditions_1: pH: 6.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
CbCACONHsample_1not availableconditions_1
2D-TOCSYsample_1not availableconditions_1

Software:

software_1 -

NMR spectrometers:

  • Varian Unity 600 MHz

Related Database Links:

BMRB 5565 7304
PDB
GB AAB50711
REF NP_999017
SP O02826
AlphaFold O02826

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks