BMRB Entry 7186

Title:
Backbone 1H Chemical Shift Assignments for Magi 5
Deposition date:
2006-06-21
Original release date:
2008-07-16
Authors:
Sabo, Jennifer; Corzo, Gerardo; Bosmans, Frank; Billen, Bert; Villegas, Elba; Tygat, Jan; Norton, Raymond
Citation:

Citation: Corzo, Gerardo; Sabo, Jennifer; Bosmans, Frank; Billen, Bert; Villegas, Elba; Tygat, Jan; Norton, Raymond. "Solution structure and alanine scan of a spider toxin that affects the activation of mammalian voltage-gated sodium channels"  J. Biol. Chem. 282, 4643-4652 (2007).
PubMed: 17148449

Assembly members:

Assembly members:
Magi 5, polymer, 29 residues, 3268.8 Da.

Natural source:

Natural source:   Common Name: Macrothele gigas   Taxonomy ID: 223896   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Macrothele gigas

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Magi 5: GCKLTFWKCKNKKECCGWNA CALGICMPR

Data sets:
Data typeCount
1H chemical shifts178
coupling constants14

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Magi 5 polypeptide1

Entities:

Entity 1, Magi 5 polypeptide 29 residues - 3268.8 Da.

1   GLYCYSLYSLEUTHRPHETRPLYSCYSLYS
2   ASNLYSLYSGLUCYSCYSGLYTRPASNALA
3   CYSALALEUGLYILECYSMETPROARG

Samples:

sample_1: Magi 5 2.2 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 4.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1not availablesample_cond_1
2D 1H-1H NOESYsample_1not availablesample_cond_1
2D 1H-1H DQF-COSYsample_1not availablesample_cond_1

Software:

xwinnmr v3.5 - processing

TOPSPIN v1.3 - processing

XEASY v1.3.13 - data analysis

CYANA v1.0.6 - structure solution

X-PLOR NIH v2.9.3 - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 800 MHz