BMRB Entry 7053

Title:
1H, 13C and 15N resonance assigned of a first cysteine catalytic half-domain of mouse ubiquitin-activating enzyme E1.
Deposition date:
2006-04-04
Original release date:
2006-06-28
Authors:
Zhukov, Igor
Citation:

Citation: Jaremko, Lukasz; Jaremko, Mariusz; Filipek, Renata; Wojciechowski, Marek; Szczepanowski, Roman; Bochtler, Matthias; Zhukov, Igor. "NMR assignment of a structurally uncharacterised fragment of recombinant mouse ubiquitin-activating enzyme"  J. Biomol. NMR 36, 43-43 (2006).
PubMed: 16738783

Assembly members:

Assembly members:
first cysteine catalytic half-domain, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts437
15N chemical shifts108
1H chemical shifts675

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mouse E1 protein1

Entities:

Entity 1, mouse E1 protein 111 residues - Formula weight is not available

1   PHEGLYGLUGLUMETVALLEUTHRASPSER
2   ASNGLYGLUGLNPROLEUSERALAMETVAL
3   SERMETVALTHRLYSASPASNPROGLYVAL
4   VALTHRCYSLEUASPGLUALAARGHISGLY
5   PHEGLUTHRGLYASPPHEVALSERPHESER
6   GLUVALGLNGLYMETILEGLNLEUASNGLY
7   CYSGLNPROMETGLUILELYSVALLEUGLY
8   PROTYRTHRPHESERILECYSASPTHRSER
9   ASNPHESERASPTYRILEARGGLYGLYILE
10   VALSERGLNVALLYSVALPROLYSLYSILE
11   SERPHELYSSERLEUPROALASERLEUVAL
12   GLU

Samples:

sample_1: first cysteine catalytic half-domain 1.0

conditions_1: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HBHA(CO)NHsample_1not availableconditions_1
HNHAsample_1not availableconditions_1
C(CO)NHsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
NOESY-HSQC 15N-editedsample_1not availableconditions_1
NOESY-HSQC 13C-editedsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Unity+ 500 MHz

Related Database Links:

BMRB 18758
PDB
DBJ BAA01433 BAC40121 BAC40405 BAE25369 BAE42599
GB AAH58630 AAH85791 AAI38201 AAI45985 EDL00743
REF NP_001014102 NP_001129557 NP_001263245 NP_001263246 NP_033483
SP Q02053 Q5U300
AlphaFold Q02053 Q5U300

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks