BMRB Entry 6965

Title:
1H and 15N assignment of the soluble domain of the ba3 oxidase subunit II of Thermus thermophilus in the oxidized state
Deposition date:
2006-02-01
Original release date:
2006-05-16
Authors:
Muresanu, Lucia; Pristovsek, Primoz; Loehr, Frank; Maneg, Oliver; Mukrasch, Marco; Rueterjans, Heinz; Ludwig, Bernd; Luecke, Christian
Citation:

Citation: Muresanu, Lucia; Pristovsek, Primoz; Loehr, Frank; Maneg, Oliver; Mukrasch, Marco; Rueterjans, Heinz; Ludwig, Bernd; Luecke, Christian. "The electron transfer complex between cytochrome c552 and the CuA domain of the Thermus thermophilus ba3 oxidase - a combined NMR and computational approach"  J. Biol. Chem. 281, 14503-14513 (2006).
PubMed: 16554303

Assembly members:

Assembly members:
CuA domain, polymer, 136 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.
CU, non-polymer, 63.546 Da.

Natural source:

Natural source:   Common Name: Thermus thermophilus   Taxonomy ID: 274   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMA10

Data sets:
Data typeCount
15N chemical shifts122
1H chemical shifts871

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CuA domain1
2COPPER (I) ION, 12
3COPPER (II) ION, 23

Entities:

Entity 1, CuA domain 136 residues - Formula weight is not available

1   METALATYRTHRLEUALATHRHISTHRALA
2   GLYVALILEPROALAGLYLYSLEUGLUARG
3   VALASPPROTHRTHRVALARGGLNGLUGLY
4   PROTRPALAASPPROALAGLNALAVALVAL
5   GLNTHRGLYPROASNGLNTYRTHRVALTYR
6   VALLEUALAPHEALAPHEGLYTYRGLNPRO
7   ASNPROILEGLUVALPROGLNGLYALAGLU
8   ILEVALPHELYSILETHRSERPROASPVAL
9   ILEHISGLYPHEHISVALGLUGLYTHRASN
10   ILEASNVALGLUVALLEUPROGLYGLUVAL
11   SERTHRVALARGTYRTHRPHELYSARGPRO
12   GLYGLUTYRARGILEILECYSASNGLNTYR
13   CYSGLYLEUGLYHISGLNASNMETPHEGLY
14   THRILEVALVALLYSGLU

Entity 2, COPPER (I) ION, 1 - Cu - 63.546 Da.

1   CU1

Entity 3, COPPER (II) ION, 2 - Cu - 63.546 Da.

1   CU

Samples:

sample_1: CuA domain, [U-98% 15N], 1.2 mM; potassium phosphate 20 mM; potassium hexacyanoferrate (III) 5 mM

sample_2: CuA domain 2.0 mM; potassium phosphate 20 mM; potassium hexacyanoferrate (III) 5 mM

condition_1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-1H TROSYnot availablenot availablecondition_1
15N-1H TOCSY-TROSYnot availablenot availablecondition_1
15N-1H NOESY-TROSYnot availablenot availablecondition_1
1H-1H TOCSYnot availablenot availablecondition_1
1H-1H NOESYnot availablenot availablecondition_1

Software:

xwinnmr v3.5 - data acquisition, data processing

AURELIA v2.5.9 - data analysis

NMR spectrometers:

  • Bruker DMX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks