BMRB Entry 6952

Title:
Violacin A
Deposition date:
2006-01-27
Original release date:
2006-04-26
Authors:
Ireland, D.; Craik, D.; Daly, N.
Citation:

Citation: Ireland, D.; Colgrave, M.; Nguyencong, P.; Daly, N.; Craik, D.. "Discovery and Characterization of a Linear Cyclotide from Viola odorata: Implications for the Processing of Circular Proteins"  J. Mol. Biol. 357, 1522-1535 (2006).
PubMed: 16488428

Assembly members:

Assembly members:
Violacin A, polymer, 27 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Viola odorata   Taxonomy ID: 97441   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Viola odorata

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Violacin A: SAISCGETCFKFKCYTPRCS CSYPVCK

Data sets:
Data typeCount
1H chemical shifts132

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Violacin A1

Entities:

Entity 1, Violacin A 27 residues - Formula weight is not available

1   SERALAILESERCYSGLYGLUTHRCYSPHE
2   LYSPHELYSCYSTYRTHRPROARGCYSSER
3   CYSSERTYRPROVALCYSLYS

Samples:

sample_1: Violacin A 1 mM; H2O 90%; D2O 10%

sample_2: Violacin A 1 mM; D2O 100%

sample_cond_1: pH: 3.8; pressure: 1 atm; temperature: 298 K

sample_cond_2: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
E-COSYnot availablenot availablenot available

Software:

xwinnmr v3.5 - processing

DYANA v1.5 - refinement

CNS v1.1 - structure solution

NMR spectrometers:

  • Bruker ARX 600 MHz

Related Database Links:

PDB
GenBank ABC94585
SWISS-PROT Q2HY54