BMRB Entry 6925

Title:
Backbone and Beta 1H, 13C, and 15N Chemical Shift Assignments for the Bacterial Toxin Kid
Deposition date:
2006-04-13
Original release date:
2006-11-06
Authors:
Kamphuis, Monique; Boelens, Rolf
Citation:

Citation: Kamphuis, Monique; Bonvin, A.; Monti, M.; Lemonnier, M.; Munoz-Gomez, A.; van den Heuvel, R.; Diaz-Orejas, R.; Boelens, Rolf. "Model for RNA binding and the catalytic site of the RNase Kid of the bacterial parD toxin-antitoxin system"  J. Mol. Biol. 357, 115-126 (2006).
PubMed: 16413033

Assembly members:

Assembly members:
Kid toxin, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts308
15N chemical shifts102
1H chemical shifts384

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Kid toxin1

Entities:

Entity 1, Kid toxin 110 residues - Formula weight is not available

1   METGLUARGGLYGLUILETRPLEUVALSER
2   LEUASPPROTHRALAGLYHISGLUGLNGLN
3   GLYTHRARGPROVALLEUILEVALTHRPRO
4   ALAALAPHEASNARGVALTHRARGLEUPRO
5   VALVALVALPROVALTHRSERGLYGLYASN
6   PHEALAARGTHRALAGLYPHEALAVALSER
7   LEUASPGLYVALGLYILEARGTHRTHRGLY
8   VALVALARGCYSASPGLNPROARGTHRILE
9   ASPMETLYSALAARGGLYGLYLYSARGLEU
10   GLUARGVALPROGLUTHRILEMETASNGLU
11   VALLEUGLYARGLEUSERTHRILELEUTHR

Samples:

sample_1: Kid toxin, [U-13C; U-15N], 0.4 mM; PO4 (K) 100 mM

conditions_1: pH: 5.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1not availableconditions_1
2D 1H-13C HSQCsample_1not availableconditions_1
3D HNCAsample_1not availableconditions_1
3D HN(CO)CAsample_1not availableconditions_1
3D HNCACBsample_1not availableconditions_1
3D CBA(CO)NHsample_1not availableconditions_1
3D HNCOsample_1not availableconditions_1
3D HA(CA)NHsample_1not availableconditions_1
3D HBA(CO)NHsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
DBJ BAA78898 BAL45864 BAL48674 BAN28644 BAP10671
EMBL CAA29585 CAB90820 CAB90822 CAD27776 CAH64731
GB AAA26070 AAO49558 AAR05736 AAR25128 AAZ03778
REF NP_052994 NP_862963 NP_957647 WP_000439432 WP_000439433
SP P13976
AlphaFold P13976

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks