BMRB Entry 6818

Title:
Structure of the cyclic conotoxin MII-6
Deposition date:
2005-09-09
Original release date:
2005-11-21
Authors:
Clark, R.; Fischer, H.; Dempster, L.; Daly, N.; Rosengren, K.; Nevin, S.; Meunier, F.; Adams, D.; Craik, D.
Citation:

Citation: Clark, R.; Fischer, H.; Dempster, L.; Daly, N.; Rosengren, K.; Nevin, S.; Meunier, F.; Adams, D.; Craik, D.. "Engineering stable peptide toxins by means of backbone cyclization: stabilization of the alpha-conotoxin MII."  Proc. Natl. Acad. Sci. U. S. A. 102, 13767-13772 (2005).
PubMed: 16162671

Assembly members:

Assembly members:
Alpha-conotoxin MII-6, polymer, 22 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Alpha-conotoxin MII-6: GCCSNPVCHLEHSNLCGGAA GG

Data sets:
Data typeCount
1H chemical shifts99

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Alpha-conotoxin MII-61

Entities:

Entity 1, Alpha-conotoxin MII-6 22 residues - Formula weight is not available

1   GLYCYSCYSSERASNPROVALCYSHISLEU
2   GLUHISSERASNLEUCYSGLYGLYALAALA
3   GLYGLY

Samples:

sample_1: Alpha-conotoxin MII-6 1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
E-COSYnot availablenot availablenot available

Software:

xwinnmr v3.5 - collection

XEASY v1.3.7 - data analysis

DYANA v1.5 - structure solution

CNS v1.1 - refinement, structure solution

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

PDB
PRF 2211312A
SWISS-PROT P56636