BMRB Entry 6811

Title:
Solution structure of apoCadA
Deposition date:
2005-09-07
Original release date:
2007-01-26
Authors:
Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Su, X.-C.; Miras, R.; Bal, N.; Mintz, E.; Catty, P.; Shokes, J.; Scott, R.
Citation:

Citation: Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Su, X.-C.; Miras, R.; Bal, N.; Mintz, E.; Catty, P.; Shokes, J.; Scott, R.. "Structural Basis for Metal Binding Specificity: the N-terminal Cadmium Binding Domain of the P1-type ATPase CadA."  J. Mol. Biol. 356, 638-650 (2005).
PubMed: 16388822

Assembly members:

Assembly members:
P-type ATPase, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Listeria monocytogenes   Taxonomy ID: 1639   Superkingdom: Eubacteria   Kingdom: not applicable   Genus/species: Listeria monocytogenes

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts75
1H chemical shifts458

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cadmium-transporting ATPase1

Entities:

Entity 1, Cadmium-transporting ATPase 71 residues - Formula weight is not available

1   METALAGLULYSTHRVALTYRARGVALASP
2   GLYLEUSERCYSTHRASNCYSALAALALYS
3   PHEGLUARGASNVALLYSGLUILEGLUGLY
4   VALTHRGLUALAILEVALASNPHEGLYALA
5   SERLYSILETHRVALTHRGLYGLUALASER
6   ILEGLNGLNVALGLUGLNALAGLYALAPHE
7   GLUHISLEULYSILEILEPROGLULYSGLU
8   ALA

Samples:

sample_1: P-type ATPase, [U-15N], 2 mM; sodium phosphate buffer 350 mM; TCEP 5 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 350 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

xwinnmr - collection, processing

NEASY - data analysis

DYANA - structure solution

AMBER v5.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
EMBL CBV37084 CBV37138 CBV37201 CCO11808 CDM15195
GB AAA25275 ADB69774 ADI61866 AGR27763 AGT06861
REF WP_003728466 WP_015076936 WP_031644883 YP_003415136 YP_003728018
SP Q60048
AlphaFold Q60048

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks