BMRB Entry 6555

Title:
Solution structure of YBL071w-A from Saccharomyces cerevisiae
Deposition date:
2005-03-18
Original release date:
2008-07-01
Authors:
Lukin, J.; Guido, V.; Arrowsmith, C.
Citation:

Citation: Proudfoot, Michael; Sanders, Stephen; Singer, Alex; Zhang, Rongguang; Brown, Greg; Binkowski, Andrew; Xu, Linda; Lukin, Jonathan; Murzin, Alexey; Joachimiak, Andrzej; Arrowsmith, Cheryl; Edwards, Aled; Savchenko, Alexei; Yakunin, Alexander. "Biochemical and Structural Characterization of a Novel Family of Cystathionine beta-Synthase Domain Proteins Fused to a Zn Ribbon-Like Domain"  J. Mol. Biol. 375, 301-315 (2008).
PubMed: 18021800

Assembly members:

Assembly members:
YBL071w-A, polymer, 82 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts451
13C chemical shifts308
15N chemical shifts79

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein YBL071w-A1
2ZINC ION2

Entities:

Entity 1, protein YBL071w-A 82 residues - Formula weight is not available

1   METSERTHRTYRASPGLUILEGLUILEGLU
2   ASPMETTHRPHEGLUPROGLUASNGLNMET
3   PHETHRTYRPROCYSPROCYSGLYASPARG
4   PHEGLNILETYRLEUASPASPMETPHEGLU
5   GLYGLULYSVALALAVALCYSPROSERCYS
6   SERLEUMETILEASPVALVALPHEASPLYS
7   GLUASPLEUALAGLUTYRTYRGLUGLUALA
8   GLYILEHISPROPROGLUPROILEALAALA
9   ALAALA

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: YBL071w-A, [U-15N; U-13C], 1 mM; NaCl 450 mM; benzamidine 1 mM; NaN3 0.01%; MOPS 10 mM; ZnSO4 0.01 mM; D2O 10%; H2O 90%

sample_2: YBL071w-A, [U-15N; U-13C], 1 mM; NaCl 450 mM; benzamidine 1 mM; NaN3 0.01%; MOPS 10 mM; ZnSO4 0.01 mM; D2O 99.9%

sample_cond_1: pH: 6.5; temperature: 298 K; ionic strength: 450 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablesample_cond_1
3D 13C-separated NOESYnot availablenot availablesample_cond_1

Software:

NMRPipe v2.1 - processing

CYANA v2.0 - structure solution

CNS v1.1 - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

REF NP_660100
SWISS-PROT Q3E840
GenBank EDN64549 EDV12157
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks