BMRB Entry 6312

Title:
Solution Structure of Fasciclin-Like Protein From Rhodobacter sphaeroides
Deposition date:
2004-09-03
Original release date:
2006-10-19
Authors:
Moody, Robert; Phillips-Jones, Mary; Williamson, Mike
Citation:

Citation: Moody, Robert; Phillips-Jones, Mary; Williamson, Mike. "NMR assignment of the Rhodobacter sphaeroides fasciclin-1 domain protein (Fdp)"  Biomol. NMR Assignments 1, 11-12 (2007).
PubMed: 19636813

Assembly members:

Assembly members:
flp, polymer, 137 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rhodobacter sphaeroides   Taxonomy ID: 1063   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Rhodobacter sphaeroides

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET14b

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts893
13C chemical shifts557
15N chemical shifts132

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1flp1

Entities:

Entity 1, flp 137 residues - Formula weight is not available

1   GLUTHRGLYASPILEVALGLUTHRALATHR
2   GLYALAGLYSERPHETHRTHRLEULEUTHR
3   ALAALAGLUALAALAGLYLEUVALASPTHR
4   LEULYSGLYASPGLYPROPHETHRVALPHE
5   ALAPROTHRASPALAALAPHEALAALALEU
6   PROGLUGLYTHRVALGLUASPLEULEULYS
7   PROGLUASNLYSGLULYSLEUTHRGLUILE
8   LEUTHRTYRHISVALVALPROGLYGLUVAL
9   METSERSERASPLEUTHRGLUGLYMETTHR
10   ALAGLUTHRVALGLUGLYGLYALALEUTHR
11   VALTHRLEUGLUGLYGLYPROLYSVALASN
12   GLYVALSERILESERGLNPROASPVALASP
13   ALASERASNGLYVALILEHISVALILEASP
14   GLYVALLEUMETPROGLYALA

Samples:

sample_1: flp, [U-13C; U-15N], 1.0 mM; sodium phosphate 50 mM

condition_1: pH: 7; temperature: 298 K; ionic strength: 0.05 M

Experiments:

NameSampleSample stateSample conditions
Standard 2D and 3D spectra for assignment and measurement of NOEsnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

FELIX -

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 800 MHz

Related Database Links:

PDB
GB ABA80588 ABN75196 ACM02667 EGJ22911 EKX58484
REF WP_002722035 WP_009563440 WP_011338938 WP_011840152 YP_001041968

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks