BMRB Entry 6263

Title:
Solution Structure of a Loop Truncated Mutant from D. gigas Rubredoxin, NMR
Deposition date:
2004-07-16
Original release date:
2005-03-15
Authors:
Pais, T.; Lamosa, P.; dos Santos, W.; LeGall, J.; Turner, D.; Santos, H.
Citation:

Citation: Pais, T.; Lamosa, P.; dos Santos, W.; LeGall, J.; Turner, D.; Santos, H.. "Structural Determinants of Protein Stabilization by Solutes: The Important of the Hair-pin Loop in Rubredoxins"  FEBS J. 272, 999-1011 (2005).
PubMed: 15691333

Assembly members:

Assembly members:
Rubredoxin, polymer, 39 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Desulfovibrio gigas   Taxonomy ID: 879   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Desulfovibrio gigas

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pT7-7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rubredoxin: MDIYVCTVCGYEYDPAFEDL PDDWACPVCGASKDAFEKQ

Data sets:
Data typeCount
1H chemical shifts219

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rubredoxin1

Entities:

Entity 1, Rubredoxin 39 residues - Formula weight is not available

1   METASPILETYRVALCYSTHRVALCYSGLY
2   TYRGLUTYRASPPROALAPHEGLUASPLEU
3   PROASPASPTRPALACYSPROVALCYSGLY
4   ALASERLYSASPALAPHEGLULYSGLN

Samples:

sample_1: Rubredoxin 4 mM; H2O 90%; D2O 10%; NaCl 20 mM

sample_cond_1: ionic strength: 20 mM; pH: 7.6; pressure: 1 atm; temperature: 303 K

sample_cond_2: ionic strength: 20 mM; pH: 7.6; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
COSYnot availablenot availablenot available

Software:

xwinnmr v3.1 - processing

DYANA v1.4 with modifications - iterative matrix relaxation

XEASY v1.3.10 - data analysis

GLOMSA - refinement

ProcheckNMR v3.4.4 - data analysis

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB