BMRB Entry 6160

Title:
Structural and biochemical evidence for disulfide bond heterogeneity in active forms of the somatomedin B domain of human vitronectin
Deposition date:
2004-03-24
Original release date:
2004-06-29
Authors:
Kamikubo, Y.; De Guzman, R.N.; Kroon, G.; Curriden, .; Neels, J.; Dawson, P.; Oldziej, S.; Jagielska, A.; Scheraga, H.A.; Loskutoff, D.J.; Dyson, H.J.
Citation:

Citation: Kamikubo, Y.; De Guzman, R.; Kroon, G.; Curriden, S.; Neels, J.; Churchill, M.; Dawson, P.; Oldziej, S.; Jagielska, A.; Scheraga, H.; Loskutoff, D.; Dyson, H.. "Disulfide Bonding Arrangements in Active Forms of the Somatomedin B Domain of Human Vitronectin"  Biochemistry 43, 6519-6534 (2004).
PubMed: 15157085

Assembly members:

Assembly members:
somatomedin B domain of vitronectin, polymer, 51 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
somatomedin B domain of vitronectin: DQESCKGRCTEGFNVDKKCQ CDELCSYYQSCCTDYTAECK PQVTRGDVFTM

Data sets:
Data typeCount
15N chemical shifts53
1H chemical shifts304

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1somatomedin B domain of vitronectin1

Entities:

Entity 1, somatomedin B domain of vitronectin 51 residues - Formula weight is not available

1   ASPGLNGLUSERCYSLYSGLYARGCYSTHR
2   GLUGLYPHEASNVALASPLYSLYSCYSGLN
3   CYSASPGLULEUCYSSERTYRTYRGLNSER
4   CYSCYSTHRASPTYRTHRALAGLUCYSLYS
5   PROGLNVALTHRARGGLYASPVALPHETHR
6   MET

Samples:

sample_1: somatomedin B domain of vitronectin, [U-15N], 0.5 mM; sodium phosphate 20 mM

Conditions_1: pH: 7.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 15N TOCSY-HSQCsample_1not availableConditions_1
3D 15N NOESY-HSQCsample_1not availableConditions_1
2D 1QF COSYsample_1not availableConditions_1
2D NOESYsample_1not availableConditions_1
2QF COSYsample_1not availableConditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 900 MHz
  • Bruker DMX 750 MHz

Related Database Links:

BMRB 15271
PDB
DBJ BAG35807
EMBL CAA26933 CAA28659 CAI29588
GenBank AAH05046 AAK60270 ABM84320 ABM87715 EAW51082
REF NP_000629 XP_001106884 XP_001146731 XP_001146795 XP_001146856
SWISS-PROT P04004

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks