BMRB Entry 6068

Title:
Solution structure of porcine peptide YY (pPYY) in solution and bound to DPC micelles
Deposition date:
2004-01-08
Original release date:
2008-07-17
Authors:
Zerbe, O.; Lerch, M.; Mayrhofer, M.
Citation:

Citation: Lerch, M.; Mayrhofer, M.; Zerbe, O.. "Structural Similarities of micelle-bound peptide YY (PYY) and neuropeptide Y (NPY) are related to their affinity profiles at the Y receptors."  J. Biol. Chem. 339, 1153-1168 (2004).
PubMed: 15178255

Assembly members:

Assembly members:
Peptide YY, polymer, 37 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pig   Taxonomy ID: 9823   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Sus scrofa

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Peptide YY: YPAKPEAPGEDASPEELSRY YASLRHYLNLVTRQRYX

Data typeCount
1H chemical shifts499
15N chemical shifts31
coupling constants62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Peptide YY1

Entities:

Entity 1, Peptide YY 37 residues - Formula weight is not available

1   TYRPROALALYSPROGLUALAPROGLYGLU
2   ASPALASERPROGLUGLULEUSERARGTYR
3   TYRALASERLEUARGHISTYRLEUASNLEU
4   VALTHRARGGLNARGTYRNH2

Samples:

sample_1: Peptide YY 2 mM; H2O 90%; D2O 10%

sample_2: Peptide YY 2 mM; D2O 99%

sample_3: Peptide YY, [U-15N], 1 mM; H2O 90%; D2O 10%

sample_4: Peptide YY 2 mM; d38-DPC 300 mM; H2O 90%; D2O 10%

sample_5: Peptide YY 2 mM; d38-DPC 300 mM; D2O 99%

sample_6: Peptide YY, [U-15N], 1 mM; d38-DPC 300 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 4.2; temperature: 301 K; pressure: 1 atm

sample_cond_2: pH: 5.5; temperature: 310 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
15N{1H}-NOEnot availablenot availablenot available

Software:

XWINNMR v3.0 - collection

XEASY v2.51 - data analysis

DYANA v1.5 - structure solution

AMBER v6 - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker AVANCE 700 MHz

Related Database Links:

BMRB 4842
PDB
DBJ BAE20675 BAE46747
GB AAA41222 AAB19752 AAG42908 AAH10821 AEY83942
PRF 1407279A
REF NP_001029252 NP_001243457 NP_663410 XP_001113958 XP_001491077
SP P10631 P68004 P68005 Q9EPS2
AlphaFold P10631 P10631 P68004 P68004 P68005 P68005 Q9EPS2 Q9EPS2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks