BMRB Entry 5870

Title:
Structural Studies of Cu(I)-Bleomycin
Deposition date:
2003-07-16
Original release date:
2004-10-25
Authors:
Lehmann, T.
Citation:

Citation: Lehmann, T.. "Structural Study of Cu(I)-Bleomycin"  J. Biol. Inorg. Chem. 9, 323-334 (2004).
PubMed: 15015041

Assembly members:

Assembly members:
BLEOMYCIN A2, non-polymer, 1416.560 Da.
CU1, non-polymer, 63.546 Da.

Natural source:

Natural source:   Common Name: Streptomyces verticillus   Taxonomy ID: 29309   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces verticillus

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BLEOMYCIN A21
2COPPER (I) ION2

Entities:

Entity 1, BLEOMYCIN A2 1 residues - C55 H85 N17 O21 S3 - 1416.560 Da.

1   BLM

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1   CU1

Samples:

sample_1: BLEOMYCIN A2 5 mM; COPPER (I) ION 5 mM; H2O 90%; D2O 10%

sample_2: BLEOMYCIN A2 5 mM; COPPER (I) ION 5 mM; D2O 100%

sample_cond_1: ionic strength: 5 mM; pH: 8.1; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1

Software:

xwinnmr v2.5 - collection

NMRPipe v2002 - processing

DISCOVER v2003 - refinement, structure solution

NMR spectrometers:

  • Bruker DRX 500 MHz