BMRB Entry 5790

Title:
Solution structure of the Hypothetical protein AQ_1857 from Aquifex aeolicus: Northeast Structural Genomics Consortium Target QR6
Deposition date:
2003-05-01
Original release date:
2004-03-15
Authors:
Xu, D.; Liu, G.; Xiao, R.; Acton, T.; Montelione, G.; Szyperski, T.
Citation:

Citation: Xu, D.; Liu, G.; Xiao, R.; Acton, T.; Goldsmith-Fischman, S.; Honig, B.; Montelione, G.; Szyperski, T.. "NMR structure of the hypothetical protein AQ-1857 encoded by the Y157 gene from Aquifex aeolicus reveals a novel protein fold. "  Proteins 54, 794-796 (2004).
PubMed: 14997575

Assembly members:

Assembly members:
Hypothetical protein AQ_1857, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Aquifex aeolicus   Taxonomy ID: 63363   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Aquifex aeolicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET21

Data sets:
Data typeCount
coupling constants50

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Glutamate receptor interacting protein1

Entities:

Entity 1, Glutamate receptor interacting protein 124 residues - Formula weight is not available

1   METGLNGLUGLNALAGLNGLNPHEILEPHE
2   LYSVALTHRASPLYSALAVALGLUGLUILE
3   LYSLYSVALALAGLNGLUASNASNILEGLU
4   ASNPROILELEUARGILEARGVALVALPRO
5   GLYGLYCYSSERGLYPHEGLNTYRALAMET
6   GLYPHEASPASPTHRVALGLUGLUGLYASP
7   HISVALPHEGLUTYRASPGLYVALLYSVAL
8   VALILEASPPROPHESERMETPROTYRVAL
9   ASNGLYALAGLULEUASPTYRVALVALASP
10   PHEMETGLYGLYGLYPHETHRILEARGASN
11   PROASNALATHRGLYSERCYSGLYCYSGLY
12   SERSERPHESERCYSGLYLEUGLUHISHIS
13   HISHISHISHIS

Samples:

sample_1: Hypothetical protein AQ_1857, [U-15N; U-13C], 1.08 mM; MES 20 mM; NaCl 50 mM; DTT 5 mM; D2O 5%; NaN3 0.02%

sample_2: Hypothetical protein AQ_1857, [U-15N; U-5% 13C], 1.01 mM; MES 20 mM; NaCl 100 mM; DTT 10 mM; D2O 5%; NaN3 0.02%

sample_cond_1: pH: 6.5; temperature: 293 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
ct-13C-HSQCnot availablenot availablenot available
3D-HNNCACBnot availablenot availablenot available
3D-RD-HABCABcoNHNnot availablenot availablenot available
3D-RD-HCCH-COSYnot availablenot availablenot available

Software:

DYANA v1.5 - structure solution

NMRPipe v1.0 - processing

XEASY v1.3.1 - data analysis

PROSA v5.0 - processing

CYANA v1.0.3 - structure solution, refinement

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

BMRB 5683
PDB
GB AAC07682
REF NP_214277 WP_010881213
SP O67709
AlphaFold O67709