BMRB Entry 5764

Title:
1H, 13C and 15N Chemical Shift Assignments for the Nuclear Factor ALY RBD Domain
Deposition date:
2003-04-07
Original release date:
2003-08-08
Authors:
Perez-Alvarado, Gabriela; Martinez-Yamout, Maria; Allen, Melissa; Grosschedl, Rudolf; Dyson, H.; Wright, Peter
Citation:

Citation: Perez-Alvarado, Gabriela; Martinez-Yamout, Maria; Allen, Melissa; Grosschedl, Rudolf; Dyson, H.; Wright, Peter. "Structure of the Nuclear Factor ALY: Insights into Post-transcriptional Regulatory and mRNA Nuclear Export Processes"  Biochemistry 42, 7348-7357 (2003).
PubMed: 12809490

Assembly members:

Assembly members:
ALY, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia Escherichia coli   Vector: pet21a

Data sets:
Data typeCount
1H chemical shifts699
13C chemical shifts419
15N chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ALY monomer1

Entities:

Entity 1, ALY monomer 106 residues - Formula weight is not available

1   SERARGPROLYSGLNLEUPROASPLYSTRP
2   GLNHISASPLEUPHEASPSERGLYPHEGLY
3   GLYGLYALAGLYVALGLUTHRGLYGLYLYS
4   LEULEUVALSERASNLEUASPPHEGLYVAL
5   SERASPALAASPILEGLNGLULEUPHEALA
6   GLUPHEGLYTHRLEULYSLYSALAALAVAL
7   HISTYRASPARGSERGLYARGSERLEUGLY
8   THRALAASPVALHISPHEGLUARGLYSALA
9   ASPALALEULYSALAMETLYSGLNTYRASN
10   GLYVALPROLEUASPGLYARGPROMETASN
11   ILEGLNLEUVALTHRSER

Samples:

sample_1: ALY, [U-15N], 0.5 mM; Tris-HCl-d8 20 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_2: ALY, [U-15N; U-13C], 0.5 mM; Tris-HCl-d8 20 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.5; temperature: 296 K; ionic strength: 100 mM; pressure: 1 atm

sample_cond_2: pH: 6.5; temperature: 289 K; ionic strength: 100 mM; pressure: 1 atm

sample_cond_3: pH: 5.9; temperature: 296 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated_NOESYnot availablenot availablenot available
3D 13C-HMQC-NOESYnot availablenot availablenot available
3D 15N/13C-NOESY-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)CBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
C(CO)NH-TOCSYnot availablenot availablenot available
HC(CO)NH-TOCSYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
CCH-COSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HNHBnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
3D 13C-separated_NOESYnot availablenot availablenot available

Software:

XWINNMR v2.1 - collection

NMRPipe v1.8, 2.1 - processing

NMRDraw v1.8, 2.1 - processing

NMRVIEW v3 - data analysis

DYANA v1.5 - structure solution

AMBER v6, 7 - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz
  • Bruker AMX 500 MHz

Related Database Links:

PDB
EMBL CAB76383 CAJ81934
GB AAC53117 AAD09608 AAH52302 AAH92013 AAI02385
REF NP_001017027 NP_001030494 NP_001089314 NP_001103072 NP_001232343
SP B5FXN8 O08583 Q28FB9 Q3T0I4 Q58EA2
TPG DAA18257
AlphaFold B5FXN8 O08583 Q28FB9 Q3T0I4 Q58EA2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks