BMRB Entry 5670

Title:
Structural Characterization of Hellethionins from helleborus purpurascens
Deposition date:
2003-01-23
Original release date:
2003-04-30
Authors:
Milbradt, Alexander; Kerek, Franz; Moroder, Luis; Renner, Christian
Citation:

Citation: Milbradt, Alexander; Kerek, Franz; Moroder, Luis; Renner, Christian. "Structural Characterization of Hellethionins from helleborus purpurascens"  Biochemistry 42, 2404-2411 (2003).
PubMed: 12600207

Assembly members:

Assembly members:
Hellethionin D, polymer, 46 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Helleborus purpurascens   Taxonomy ID: 171899   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Helleborus purpurascens

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hellethionin D: KSCCRNTLARNCYNACRFTG GSQPTCGILCDCIHVTTTTC PSSHPS

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts291

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hellethionin D1

Entities:

Entity 1, Hellethionin D 46 residues - Formula weight is not available

1   LYSSERCYSCYSARGASNTHRLEUALAARG
2   ASNCYSTYRASNALACYSARGPHETHRGLY
3   GLYSERGLNPROTHRCYSGLYILELEUCYS
4   ASPCYSILEHISVALTHRTHRTHRTHRCYS
5   PROSERSERHISPROSER

Samples:

sample_1: Hellethionin D 3.0 mM

sample_2: Hellethionin D 5.0 mM

cond_1: pH: 2.5; temperature: 293 K

cond_2: pH: 2.5; temperature: 283 K

cond_3: pH: 2.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
1H-1H COSYnot availablenot availablenot available

Software:

XWINNMR v3.0 - data acquisition, data processing

INSIGHT v98 - structure calculation

Sparky v3 - peak assignment

MOLMOL - visualization

PROCHECK - checking the quality of the protein structure

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

PDB
SP P60057
AlphaFold P60057