BMRB Entry 52010

Title:
SARS-CoV-2 Nucleocapsid protein NTD Y109A mutant
Deposition date:
2023-06-30
Original release date:
2023-11-15
Authors:
Estelle, Aidan; Forsythe, Heather; Yu, Zhen; Hughes, Kaitlyn; Lasher, Brittany; Allen, Patrick; Reardon, Patrick; Hendrix, David; Barbar, Elisar
Citation:

Citation: Estelle, Aidan; Forsythe, Heather; Yu, Zhen; Hughes, Kaitlyn; Lasher, Brittany; Allen, Patrick; Reardon, Patrick; Hendrix, David; Barbar, Elisar. "RNA structure and multiple weak interactions balance the interplay between RNA binding and phase separation of SARS-CoV-2 nucleocapsid"  PNAS Nexus 2, pgad333-pgad333 (2023).
PubMed: 37901441

Assembly members:

Assembly members:
entity_1, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24d

Data sets:
Data typeCount
15N chemical shifts106
1H chemical shifts106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NTD Y109A1

Entities:

Entity 1, NTD Y109A 132 residues - Formula weight is not available

N-terminal domain of the nucleocapsid with a Y-to-A mutation at residue 109.

1   GLYALAHISMETASNASNTHRALASERTRP
2   PHETHRALALEUTHRGLNHISGLYLYSGLU
3   ASPLEULYSPHEPROARGGLYGLNGLYVAL
4   PROILEASNTHRASNSERSERPROASPASP
5   GLNILEGLYTYRTYRARGARGALATHRARG
6   ARGILEARGGLYGLYASPGLYLYSMETLYS
7   ASPLEUSERPROARGTRPALAPHETYRTYR
8   LEUGLYTHRGLYPROGLUALAGLYLEUPRO
9   TYRGLYALAASNLYSASPGLYILEILETRP
10   VALALATHRGLUGLYALALEUASNTHRPRO
11   LYSASPHISILEGLYTHRARGASNPROALA
12   ASNASNALAALAILEVALLEUGLNLEUPRO
13   GLNGLYTHRTHRLEUPROLYSGLYPHETYR
14   ALAGLU

Samples:

sample_1: NTD Y109A, [U-100% 15N], 500 uM; sodium chloride 20 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0.04 M; pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRViewJ v9.2.0-b27 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks