BMRB Entry 5199

Title:
Assignment of the 1H and 15N resonances of the elicitor protein NIP1
Deposition date:
2001-11-02
Original release date:
2003-06-09
Authors:
van't Slot, Klaas; van den Burg, Harrold; Kloks, Cathelijne; Hilbers, Cornelis; Knogge, Wolfgang; Papavoine, Christina
Citation:

Citation: van't Slot, Klaas; van den Burg, Harrold; Kloks, Cathelijne; Hilbers, Cornelis; Knogge, Wolfgang; Papavoine, Christina. "Solution structure of the fungal plant disease resistance-triggering protein NIP1 shows a novel b sheet fold"  .

Assembly members:

Assembly members:
Necrosis Inducing Protein 1, polymer, 60 residues, 6434 Da.

Natural source:

Natural source:   Common Name: barley leaf scald fungus   Taxonomy ID: 38038   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Rhynchosporium secalis

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Necrosis Inducing Protein 1: DRCRYTLCCDGALKAVSACL HESESCLVPGDCCRGKSRLT LCSYGEGGNGFQCPTGYRQC

Data sets:
Data typeCount
15N chemical shifts65
1H chemical shifts369
coupling constants35

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NIP11

Entities:

Entity 1, NIP1 60 residues - 6434 Da.

1   ASPARGCYSARGTYRTHRLEUCYSCYSASP
2   GLYALALEULYSALAVALSERALACYSLEU
3   HISGLUSERGLUSERCYSLEUVALPROGLY
4   ASPCYSCYSARGGLYLYSSERARGLEUTHR
5   LEUCYSSERTYRGLYGLUGLYGLYASNGLY
6   PHEGLNCYSPROTHRGLYTYRARGGLNCYS

Samples:

sample-1: Necrosis Inducing Protein 1, [U-15N], 2.0 mM

conditions1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-1H CLEAN-TOCSYnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
2D HMQCJnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D 1H-15N-15N NOESYnot availablenot availablenot available
3D 1H-1H-15N TOCSYnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNHBnot availablenot availablenot available

Software:

NMRPipe -

XEASY -

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAA86496 AAS84297 AAS84298 AAS84299 AAS84300

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks