BMRB Entry 51896

Title:
Structure of a new ShKT peptide from the sea anemone Telmatactis stephensoni: ShKT-Ts1.
Deposition date:
2023-04-02
Original release date:
2023-10-09
Authors:
Sanches, Karoline; Olushola-Siedoks, Abisola; Wai, Dorothy; Norton, Raymond
Citation:

Citation: Sanches, Karoline; Ashwood, Lauren; Olushola-Siedoks, Abisola Ave-Maria; Wai, Dorothy; Rahman, Arfatur; Shakeel, Kashmala; Naseem, Muhammad Umair; Panyi, Gyorgy; Prentis, Peter; Norton, Raymond. "Structure-function relationships in ShKT domain peptides: ShKT-Ts1 from the sea anemone Telmatactis stephensoni"  Proteins 92, 192-205 (2024).
PubMed: 37794633

Assembly members:

Assembly members:
entity_1, polymer, 37 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Telmatactis stephensoni   Taxonomy ID: 2835637   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Telmatactis stephensoni

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GACENNFSDRECERRKKDCD SSMKFRELSCPKTCGTC

Data sets:
Data typeCount
13C chemical shifts110
15N chemical shifts41
1H chemical shifts232

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ShKT_Ts11

Entities:

Entity 1, ShKT_Ts1 37 residues - Formula weight is not available

1   GLYALACYSGLUASNASNPHESERASPARG
2   GLUCYSGLUARGARGLYSLYSASPCYSASP
3   SERSERMETLYSPHEARGGLULEUSERCYS
4   PROLYSTHRCYSGLYTHRCYS

Samples:

sample_1: ShKT_Ts1, [U-99% 15N], 307 uM

sample_2: ShKT_Ts1 307 uM

sample_conditions_1: ionic strength: 0 M; pH: 5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0 M; pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_2
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
2D 1H-13C HSQC/HMQCsample_2isotropicsample_conditions_2

Software:

ARIA2 v2.3 - structure solution

TOPSPIN v3.2 - collection

CcpNMR v2.4.2 - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks