BMRB Entry 51443

Title:
Solution NMR backbone chemical shift assignment for Tetrahymena p50 peptide
Deposition date:
2022-05-14
Original release date:
2022-07-13
Authors:
He, Yao; Song, He; Chan, Henry; Wang, Yaqiang; Liu, Baocheng; Susac, Lukas; Zhou, Hong; Feigon, Juli
Citation:

Citation: He, Yao; Song, He; Chan, Henry; Liu, Baocheng; Wang, Yaqiang; Susac, Lukas; Zhou, Hong; Feigon, Juli. "Structure of Tetrahymena telomerase-bound CST with polymerase a-primase"  Nature 608, 813-818 (2022).
PubMed: 35831498

Assembly members:

Assembly members:
entity_1, polymer, 24 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Tetrahymena thermophila   Taxonomy ID: 5911   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Tetrahymena thermophila

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETduet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GCQQDDFGDGCLLQIVNYTH QSLK

Data sets:
Data typeCount
13C chemical shifts39
15N chemical shifts20
1H chemical shifts20

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tetrahymena p501

Entities:

Entity 1, Tetrahymena p50 24 residues - Formula weight is not available

1   GLYCYSGLNGLNASPASPPHEGLYASPGLY
2   CYSLEULEUGLNILEVALASNTYRTHRHIS
3   GLNSERLEULYS

Samples:

sample_1: Tetrahymena p50 peptide, [U-13C; U-15N], 0.5 mM; TRIS 20 mM; sodium chloride 50 mM; sodium azide 3 mM; TCEP 1 mM

sample_conditions_1: ionic strength: 0.07 M; pH: 7.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY v1.414 - chemical shift assignment

TOPSPIN v4.1 - collection, processing

TOPSPIN v3.5 - collection, processing

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz
  • Bruker AVANCE III HD 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks