BMRB Entry 51362

Title:
Structural basis for tRNA recognition and regulation of METTL1-WDR4 methyltransferase
Deposition date:
2022-03-14
Original release date:
2023-01-13
Authors:
Viennet, Thibault; Li, Jiazhi; Gregory, Richard; Arthanari, Haribabu
Citation:

Citation: Li, Jiazhi; Wang, Longfei; Hahn, Quentin; Nowak, Radoslaw; Viennet, Thibault; Oreliana, Esteban; Burman, Shourya; Yue, Hong; Hunkeler, Moritz; Fontana, Pietro; Wu, Hao; Arthanari, Haribabu; Fischer, Eric; Gregory, Richard. "Structural basis of regulated m7G tRNA modification by METTL1-WDR4"  Nature 613, 391-397 (2023).
PubMed: 36599985

Assembly members:

Assembly members:
entity_1, polymer, 81 residues, 9426.37 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet-1

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts193
15N chemical shifts60
1H chemical shifts60

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1METTL1 1-751

Entities:

Entity 1, METTL1 1-75 81 residues - 9426.37 Da.

1   SERGLNASPPROASNSERMETALAALAGLU
2   THRARGASNVALALAGLYALAGLUALAPRO
3   PROPROGLNLYSARGTYRTYRARGGLNARG
4   ALAHISSERASNPROMETALAASPHISTHR
5   LEUARGTYRPROVALLYSPROGLUGLUMET
6   ASPTRPSERGLULEUTYRPROGLUPHEPHE
7   ALAPROLEUTHRGLNASNGLNSERHISASP
8   ASPPROLYSASPLYSLYSGLULYSARGALA
9   GLN

Samples:

sample_1: METTL1 1-75, [U-100% 13C; U-100% 15N], 420 mM; NaPi 50 mM; NaCl 100 mM; TCEP 1 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 700 MHz

Related Database Links:

HUGO HGNC:7030

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks