BMRB Entry 50423

Title:
Chemical shifts assignments of synaptobrevin-2 in trans-SNARE complex by solid-state NMR
Deposition date:
2020-08-04
Original release date:
2023-05-04
Authors:
Zhang, Yan; Hu, YaRu; Yang, Jun; Ma, Cong
Citation:

Citation: Zhang, Yan; Hu, YaRu. "Phospholipid bilayer-based study of synaptobrevin-2 by Solid-state NMR"  .

Assembly members:

Assembly members:
entity_1, polymer, 116 residues, Formula weight is not available
entity_2, polymer, 252 residues, Formula weight is not available
entity_3, polymer, 206 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-KG

Data sets:
Data typeCount
13C chemical shifts262
15N chemical shifts69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Trans SNARE Complex, chain 11
2Trans SNARE Complex, chain 22
3Trans SNARE Complex, chain 33

Entities:

Entity 1, Trans SNARE Complex, chain 1 116 residues - Formula weight is not available

1   METSERALATHRALAALATHRVALPROPRO
2   ALAALAPROALAGLYGLUGLYGLYPROPRO
3   ALAPROPROPROASNLEUTHRSERASNARG
4   ARGLEUGLNGLNTHRGLNALAGLNVALASP
5   GLUVALVALASPILEMETARGVALASNVAL
6   ASPLYSVALLEUGLUARGASPGLNLYSLEU
7   SERGLULEUASPASPARGALAASPALALEU
8   GLNALAGLYALASERGLNPHEGLUTHRSER
9   ALAALALYSLEULYSARGLYSTYRTRPTRP
10   LYSASNLEULYSMETMETILEILELEUGLY
11   VALILECYSALAILEILELEUILEILEILE
12   ILEVALTYRPHESERTHR

Entity 2, Trans SNARE Complex, chain 2 252 residues - Formula weight is not available

1   LYSASPARGTHRGLNGLULEUARGTHRALA
2   LYSASPSERASPASPASPASPASPVALTHR
3   VALTHRVALASPARGASPARGPHEMETASP
4   GLUPHEPHEGLUGLNVALGLUGLUILEARG
5   GLYPHEILEASPLYSILEALAGLUASNVAL
6   GLUGLUVALLYSARGLYSHISSERALAILE
7   LEUALASERPROASNPROASPGLULYSTHR
8   LYSGLUGLULEUGLUGLULEUMETSERASP
9   ILELYSLYSTHRALAASNLYSVALARGSER
10   LYSLEULYSSERILEGLUGLNSERILEGLU
11   GLNGLUGLUGLYLEUASNARGSERSERALA
12   ASPLEUARGILEARGLYSTHRGLNHISSER
13   THRLEUSERARGLYSPHEVALGLUVALMET
14   SERGLUTYRASNALATHRGLNSERASPTYR
15   ARGGLUARGCYSLYSGLYARGILEGLNARG
16   GLNLEUGLUILETHRGLYARGTHRTHRTHR
17   SERGLUGLULEUGLUASPMETLEUGLUSER
18   GLYASNPROALAILEPHEALASERGLYILE
19   ILEMETASPSERSERILESERLYSGLNALA
20   LEUSERGLUILEGLUTHRARGHISSERGLU
21   ILEILELYSLEUGLUASNSERILEARGGLU
22   LEUHISASPMETPHEMETASPMETALAMET
23   LEUVALGLUSERGLNGLYGLUMETILEASP
24   ARGILEGLUTYRASNVALGLUHISALAVAL
25   ASPTYRVALGLUARGALAVALSERASPTHR
26   LYSLYS

Entity 3, Trans SNARE Complex, chain 3 206 residues - Formula weight is not available

1   METALAGLUASPALAASPMETARGASNGLU
2   LEUGLUGLUMETGLNARGARGALAASPGLN
3   LEUALAASPGLUSERLEUGLUSERTHRARG
4   ARGMETLEUGLNLEUVALGLUGLUSERLYS
5   ASPALAGLYILEARGTHRLEUVALMETLEU
6   ASPGLUGLNGLYGLUGLNLEUASPARGVAL
7   GLUGLUGLYMETASNHISILEASNGLNASP
8   METLYSGLUALAGLULYSASNLEULYSASP
9   LEUGLYLYSCYSCYSGLYLEUPHEILECYS
10   PROCYSASNLYSLEULYSSERSERASPALA
11   TYRLYSLYSALATRPGLYASNASNGLNASP
12   GLYVALVALALASERGLNPROALAARGVAL
13   VALASPGLUARGGLUGLNMETALAILESER
14   GLYGLYPHEILEARGARGVALTHRASNASP
15   ALAARGGLUASNGLUMETASPGLUASNLEU
16   GLUGLNVALSERGLYILEILEGLYASNLEU
17   ARGHISMETALALEUASPMETGLYASNGLU
18   ILEASPTHRGLNASNARGGLNILEASPARG
19   ILEMETGLULYSALAASPSERASNLYSTHR
20   ARGILEASPGLUALAASNGLNARGALATHR
21   LYSMETLEUGLYSERGLY

Samples:

sample_1: H2O 30 % w/w; synaptobrevin-2, [U-100% 13C; U-100% 15N], 18 % w/w; lipid 6 % w/w; SNAP-25 23 % w/w; Syx-1A 23 % w/w

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
3D NCACXsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1
3D CONCAsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 18.8 T MHz