BMRB Entry 50389

Title:
Backbone Assignments of the Rec3 domain of CRISPR-Cas9 for S. pyogenes
Deposition date:
2020-07-13
Original release date:
2020-09-17
Authors:
Skeens, Erin; East, Kyle; Lisi, George
Citation:

Citation: Skeens, Erin; East, Kyle; Lisi, George. "1H, 13C, 15N backbone resonance assignment of the recognition lobe subdomain 3 (Rec3) from Streptococcus pyogenes CRISPR-Cas9"  Biomol. NMR Assignments 15, 25-28 (2021).
PubMed: 32935194

Assembly members:

Assembly members:
entity_1, polymer, 218 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Streptococcus pyogenes   Taxonomy ID: 1314   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus pyogenes

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts583
15N chemical shifts200
1H chemical shifts200

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1spyRec31

Entities:

Entity 1, spyRec3 218 residues - Formula weight is not available

1   SERASNPHEASPLYSASNLEUPROASNGLU
2   LYSVALLEUPROLYSHISSERLEULEUTYR
3   GLUTYRPHETHRVALTYRASNGLULEUTHR
4   LYSVALLYSTYRVALTHRGLUGLYMETARG
5   LYSPROALAPHELEUSERGLYGLUGLNLYS
6   LYSALAILEVALASPLEULEUPHELYSTHR
7   ASNARGLYSVALTHRVALLYSGLNLEULYS
8   GLUASPTYRPHELYSLYSILEGLUCYSPHE
9   ASPSERVALGLUILESERGLYVALGLUASP
10   ARGPHEASNALASERLEUGLYTHRTYRHIS
11   ASPLEULEULYSILEILELYSASPLYSASP
12   PHELEUASPASNGLUGLUASNGLUASPILE
13   LEUGLUASPILEVALLEUTHRLEUTHRLEU
14   PHEGLUASPARGGLUMETILEGLUGLUARG
15   LEULYSTHRTYRALAHISLEUPHEASPASP
16   LYSVALMETLYSGLNLEULYSARGARGARG
17   TYRTHRGLYTRPGLYARGLEUSERARGLYS
18   LEUILEASNGLYILEARGASPLYSGLNSER
19   GLYLYSTHRILELEUASPPHELEULYSSER
20   ASPGLYPHEALAASNARGASNPHEMETGLN
21   LEUILEHISASPASPSERLEUTHRPHELYS
22   GLUASPILEGLNLYSALAGLNVAL

Samples:

sample_1: spyRec3, [U-13C; U-15N; U-2H], 1.2 mM; potassium chloride 80 mM; HEPES 20 mM

sample_conditions_1: ionic strength: 80 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMRPipe - processing

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks