BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 50185

Title: AcrIIA5   PubMed: 32544231

Deposition date: 2020-01-29 Original release date: 2020-06-03

Authors: Suh, Jeong-Yong

Citation: An, So Young; Ka, Donghyun; Kim, Iktae; Kim, Eun-Hee; Kim, Nak-Kyoon; Bae, Euiyoung; Suh, Jeong-Yong. "Intrinsic disorder is essential for Cas9 inhibition of anti-CRISPR AcrIIA5"  Nucleic Acids Res. ., .-. (2020).

Assembly members:
Anti-CRISPR_AcrIIA5, polymer, 140 residues, Formula weight is not available

Natural source:   Common Name: Streptococcus thermophilus   Taxonomy ID: 1308   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus thermophilus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Anti-CRISPR_AcrIIA5: MAYGKSRYNSYRKRSFNRSN KQRREYAQEMDRLEKAFENL DGWYLSSMKDSAYKDFGKYE IRLSNHSADNKYHDLENGRL IVNIKASKLNFVDIIENKLD KIIEKIDKLDLDKYRFINAT NLEHDIKCYYKGFKTKKEVI

Data sets:
Data typeCount
13C chemical shifts533
15N chemical shifts121
1H chemical shifts795

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1AcrIIA51

Entities:

Entity 1, AcrIIA5 140 residues - Formula weight is not available

1   METALATYRGLYLYSSERARGTYRASNSER
2   TYRARGLYSARGSERPHEASNARGSERASN
3   LYSGLNARGARGGLUTYRALAGLNGLUMET
4   ASPARGLEUGLULYSALAPHEGLUASNLEU
5   ASPGLYTRPTYRLEUSERSERMETLYSASP
6   SERALATYRLYSASPPHEGLYLYSTYRGLU
7   ILEARGLEUSERASNHISSERALAASPASN
8   LYSTYRHISASPLEUGLUASNGLYARGLEU
9   ILEVALASNILELYSALASERLYSLEUASN
10   PHEVALASPILEILEGLUASNLYSLEUASP
11   LYSILEILEGLULYSILEASPLYSLEUASP
12   LEUASPLYSTYRARGPHEILEASNALATHR
13   ASNLEUGLUHISASPILELYSCYSTYRTYR
14   LYSGLYPHELYSTHRLYSLYSGLUVALILE

Samples:

sample_1_AcrIIA5: entity_1, [U-100% 13C; U-100% 15N], 0.7 mM

sample_conditions_1: ionic strength: 0.5 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESYsample_1_AcrIIA5isotropicsample_conditions_1

Software:

PIPP, Garrett - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts