BMRB Entry 50118

Title:
Resonance assignment of human STIM1 CC1 R304W mutant
Deposition date:
2019-12-06
Original release date:
2020-09-05
Authors:
Rathner, Petr; Mueller, Norbert; Cerofolini, Linda; Ravera, Enrico; Luchinat, Claudio
Citation:

Citation: Rathner, Petr; Fahrner, Marc; Cerofolini, Linda; Grabmayr, Herwig; Horvath, Ferdinand; Krobath, Heinrich; Gupta, Agrim; Ravera, Enrico; Fragai, Marco; Bechmann, Matthias; Renger, Thomas; Luchinat, Claudio; Romanin, Christoph; Muller, Norbert. "Interhelical interactions within the STIM1 CC1 domain modulate CRAC channel activation"  Nat. Chem. Biol. 17, 196-204 (2021).
PubMed: 33106661

Assembly members:

Assembly members:
entity_1, polymer, 115 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX 4T-1

Data sets:
Data typeCount
13C chemical shifts295
15N chemical shifts93
1H chemical shifts369

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1STIM1 CC1 R304W1

Entities:

Entity 1, STIM1 CC1 R304W 115 residues - Formula weight is not available

1   GLYSERPROGLUPHEASNARGTYRSERLYS
2   GLUHISMETLYSLYSMETMETLYSASPLEU
3   GLUGLYLEUHISARGALAGLUGLNSERLEU
4   HISASPLEUGLNGLUARGLEUHISLYSALA
5   GLNGLUGLUHISARGTHRVALGLUVALGLU
6   LYSVALHISLEUGLULYSLYSLEUARGASP
7   GLUILEASNLEUALALYSGLNGLUALAGLN
8   ARGLEULYSGLULEUTRPGLUGLYTHRGLU
9   ASNGLUARGSERARGGLNLYSTYRALAGLU
10   GLUGLULEUGLUGLNVALARGGLUALALEU
11   ARGLYSALAGLULYSGLULEUGLUSERHIS
12   SERSERTRPTYRALA

Samples:

sample_1: TRIS 20 mM; SDS, [U-99% 2H], 7 mM; STIM1 CC1 R304W, [U-95% 13C; U-95% 15N], 0.3 mM; H2O, [U-99% 2H], 10 % w/v; H2O 90 % w/v

sample_conditions_1: ionic strength: 0.02 M; pH: 7.25; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
CONsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CARA v1.8.4.2, Keller and Wuthrich - chemical shift assignment

TOPSPIN v3.5, 3.6, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 950 MHz
  • Bruker Avance 900 MHz
  • Bruker Avance III 700 MHz
  • Bruker Avance NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks