Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR50093
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Citation: Millana, Elisa; Todesca, Sofia; Sicorello, Alessandro; Masino, Laura; Pompach, Petr; Magnani, Francesca; Pastore, Annalisa; Mattevi, Andrea. "On the mechanism of calcium-dependent activation of NADPH oxidase 5 (NOX5)" FEBS J. 287, 2486-2503 (2020).
PubMed: 31785178
Assembly members:
entity_1, polymer, . residues, 9715.906 Da.
entity_CA, non-polymer, 40.078 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: E. coli Vector: pET28 a+
Entity Sequences (FASTA):
entity_1: GDKLKFLFQVYDIDGSGSID
PDELRTVLQSCLRESAISLP
DEKLDQLTLALFESADADGN
GAITFEELRDELQRFPGVME
NLTISAAA
Data type | Count |
13C chemical shifts | 137 |
15N chemical shifts | 66 |
1H chemical shifts | 66 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | C-lobe of EF-domqin, hNOX5beta | 1 |
2 | Ca2+, 1 | 2 |
3 | Ca2+, 2 | 2 |
Entity 1, C-lobe of EF-domqin, hNOX5beta - 9715.906 Da.
The first amino acid (G) and the last three (AAA) where introduce due to cloning needs.
1 | GLY | ASP | LYS | LEU | LYS | PHE | LEU | PHE | GLN | VAL | ||||
2 | TYR | ASP | ILE | ASP | GLY | SER | GLY | SER | ILE | ASP | ||||
3 | PRO | ASP | GLU | LEU | ARG | THR | VAL | LEU | GLN | SER | ||||
4 | CYS | LEU | ARG | GLU | SER | ALA | ILE | SER | LEU | PRO | ||||
5 | ASP | GLU | LYS | LEU | ASP | GLN | LEU | THR | LEU | ALA | ||||
6 | LEU | PHE | GLU | SER | ALA | ASP | ALA | ASP | GLY | ASN | ||||
7 | GLY | ALA | ILE | THR | PHE | GLU | GLU | LEU | ARG | ASP | ||||
8 | GLU | LEU | GLN | ARG | PHE | PRO | GLY | VAL | MET | GLU | ||||
9 | ASN | LEU | THR | ILE | SER | ALA | ALA | ALA |
Entity 2, Ca2+, 1 - Ca - 40.078 Da.
1 | CA |
sample_1: C-term lobe of hNOX5beta: residues 78-161, [U-100% 13C; U-100% 15N], 200 uM; HEPES pH 7 50 mM; NaCl 25 mM; D2O 5 % v/v; CaCl2 1 mM
sample_conditions_1: ionic strength: 25 mM; pH: 7; pressure: 1 atm; temperature: 300 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
HSQC | sample_1 | isotropic | sample_conditions_1 |
HNCACB | sample_1 | isotropic | sample_conditions_1 |
HNcoCANH | sample_1 | isotropic | sample_conditions_1 |
TOPSPIN, Bruker Biospin - Data collection
NMRPipe, Delaglio, Zhengrong and Bax - processing
NMRDraw, Delaglio, Zhengrong and Bax - processing
CcpNmr_Analysis, CCPN - chemical shift assignment and data analysis
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks