BMRB Entry 50085

Title:
Collective exchange processes reveal an active site proton cage in bacteriorhodopsin
Deposition date:
2019-11-11
Original release date:
2020-01-06
Authors:
Friedrich, Daniel
Citation:

Citation: Friedrich, Daniel; Brunig, Florian; Nieuwkoop, Andrew; Netz, Roland; Hegemann, Peter; Oschkinat, Hartmut. "Collective exchange processes reveal an active site proton cage in bacteriorhodopsin"  Commun. Biol. 3, 4-4 (2020).
PubMed: 31925324

Assembly members:

Assembly members:
entity_1, polymer, 248 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Halobacterium salinarum   Taxonomy ID: 2242   Superkingdom: Archaea   Kingdom: not available   Genus/species: Halobacterium salinarum

Experimental source:

Experimental source:   Production method: native organism   Host organism: Halobacterium salinarum

Data sets:
Data typeCount
13C chemical shifts2
15N chemical shifts42
1H chemical shifts44

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bacteriorhodopsin1

Entities:

Entity 1, bacteriorhodopsin 248 residues - Formula weight is not available

1   GLNALAGLNILETHRGLYARGPROGLUTRP
2   ILETRPLEUALALEUGLYTHRALALEUMET
3   GLYLEUGLYTHRLEUTYRPHELEUVALLYS
4   GLYMETGLYVALSERASPPROASPALALYS
5   LYSPHETYRALAILETHRTHRLEUVALPRO
6   ALAILEALAPHETHRMETTYRLEUSERMET
7   LEULEUGLYTYRGLYLEUTHRMETVALPRO
8   PHEGLYGLYGLUGLNASNPROILETYRTRP
9   ALAARGTYRALAASPTRPLEUPHETHRTHR
10   PROLEULEULEULEUASPLEUALALEULEU
11   VALASPALAASPGLNGLYTHRILELEUALA
12   LEUVALGLYALAASPGLYILEMETILEGLY
13   THRGLYLEUVALGLYALALEUTHRLYSVAL
14   TYRSERTYRARGPHEVALTRPTRPALAILE
15   SERTHRALAALAMETLEUTYRILELEUTYR
16   VALLEUPHEPHEGLYPHETHRSERLEUALA
17   GLUSERMETARGPROGLUVALALASERTHR
18   PHELYSVALLEUARGASNVALTHRVALVAL
19   LEUTRPSERALATYRPROVALVALTRPLEU
20   ILEGLYSERGLUGLYALAGLYILEVALPRO
21   LEUASNILEGLUTHRLEULEUPHEMETVAL
22   LEUASPVALSERALALYSVALGLYPHEGLY
23   LEUILELEULEUARGSERARGALAILEPHE
24   GLYGLUALAGLUALAPROGLUPROSERALA
25   GLYASPGLYALAALAALATHRSER

Samples:

sample_1: bacteriorhodopsin, DCN, 10 mM

sample_conditions_1: pH: 7.5; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CcpNMR, CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks