BMRB Entry 50078

Title:
1H, 15N and 13C assignment of BRCA2 53-131
Deposition date:
2019-10-23
Original release date:
2019-10-29
Authors:
Julien, Manon; Miron, Simona; Carreira, Aura; Theillet, Francois-Xavier; Zinn-Justin, Sophie
Citation:

Citation: Julien, Manon; Miron, Simona; Carreira, Aura; Theillet, Francois-Xavier; Zinn-Justin, Sophie. "1H, 13C and 15N backbone resonance assignment of the human BRCA2 N-terminal region"  Biomol. NMR Assignments 14, 79-85 (2020).
PubMed: 31900740

Assembly members:

Assembly members:
entity_1, polymer, 85 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-41b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts215
15N chemical shifts68
1H chemical shifts68

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BRCA2 53-1311

Entities:

Entity 1, BRCA2 53-131 85 residues - Formula weight is not available

1   GLYGLUGLUSERGLUHISLYSASNASNASN
2   TYRGLUPROASNLEUPHELYSTHRPROGLN
3   ARGLYSPROSERTYRASNGLNLEUALASER
4   THRPROILEILEPHELYSGLUGLNGLYLEU
5   THRLEUPROLEUTYRGLNSERPROVALLYS
6   GLULEUASPLYSPHELYSLEUASPLEUGLY
7   ARGASNVALPROASNSERARGHISLYSSER
8   LEUARGTHRVALLYSTHRLYSMETASPGLN
9   ALAASPASPVALSER

Samples:

sample_1: brca2 53-131, [U-95% 13C; U-95% 15N], 0.2 mM; HEPES 50 mM; EDTA 1 mM; DTT 2 mM; DSS 50 uM; D2O, [U-99% 2H], 10%

sample_conditions_1: ionic strength: 0 M; pH: 7.0; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D HSQCsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4, Bruker Biospin, CCPN - chemical shift assignment, collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP P51587
AlphaFold Q5TBJ7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks