BMRB Entry 50023

Name: sf1c2
Published: 2020-09-21

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50023

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

sf1c2

Deposition date:

2019-09-23

Original release date:

2020-09-21

Authors:

Wiesner, Silke

Citation:

Citation: Ruetalo, Natalia; Anders, Samira; Stollmaier, Carsten; Jaeckl, Magnus; Schuetz-Stoffregen, Mira; Stefan, Nadine; Wolf, Christine; Wiesner, Silke. "The WW1 Domain Enhances Autoinhibition in Smurf Ubiquitin Ligases" J. Mol. Biol. 431, 4834-4847 (2019).
PubMed: 31628949

Assembly members:

Assembly members:
Sf1C2, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETZ

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sf1C2: GAMGSSIKIRLTVLCAKNLA KKDFFRLPDPFAKIVVDGSG QCHSTDTVKNTLDPKWNQHY DLYVGKTDSITISVWNHKKI HKKQGAGFLGCVRLLSNAIS RLKDTGYQRLDLCKLNPSDT DAVRGQIVVSLQTR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	227
15N chemical shifts	117
1H chemical shifts	117

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	sf1c2	1

Entities:

Entity 1, sf1c2 134 residues - Formula weight is not available

1

GLY

ALA

MET

GLY

SER

ILE

LYS

ILE

ARG

2

LEU

THR

VAL

LEU

CYS

ALA

LYS

ASN

LEU

ALA

3

LYS

ASP

PHE

ARG

LEU

PRO

ASP

PRO

4

PHE

ALA

LYS

ILE

VAL

ASP

GLY

SER

GLY

5

GLN

CYS

HIS

SER

THR

ASP

THR

VAL

LYS

ASN

6

THR

LEU

ASP

PRO

LYS

TRP

ASN

GLN

HIS

TYR

7

ASP

LEU

TYR

VAL

GLY

LYS

THR

ASP

SER

ILE

8

THR

ILE

SER

VAL

TRP

ASN

HIS

LYS

ILE

9

HIS

LYS

GLN

GLY

ALA

GLY

PHE

LEU

GLY

10

CYS

VAL

ARG

LEU

SER

ASN

ALA

ILE

SER

11

ARG

LEU

LYS

ASP

THR

GLY

TYR

GLN

ARG

LEU

12

ASP

LEU

CYS

LYS

LEU

ASN

PRO

SER

ASP

THR

13

ASP

ALA

VAL

ARG

GLY

GLN

ILE

VAL

SER

14

LEU

GLN

THR

ARG

Samples:

sample_1: Smurf1 C2 domain, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium azide 150 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.5 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks