BMRB Entry 4633

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PDB ID: 1fuw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4633
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure, Backbone Dynamics, and stability of a Double Mutant Single-Chain Monellin. Structural origin of sweetness

Deposition date:

2000-09-18

Original release date:

2002-12-23

Authors:

Sung, Y.; Shin, J.; Chang, H.; Cho, J.; Lee, W.

Citation:

Citation: Sung, Y.; Shin, J.; Chang, H.; Cho, J.; Lee, W.. "Solution Structure, Backbone Dynamics, and stability of a Double Mutant Single-Chain Monellin. Structural origin of sweetness " J. Biol. Chem. 276, 19624-19630 (2001).
PubMed: 11279156

Assembly members:

Assembly members:
MONELLIN, polymer, 91 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Serendipity berry Taxonomy ID: 3457 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Dioscoreophyllum cumminsii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MONELLIN: GEWEIIEIGPFYQNLGKFAV DEENKIGQYGRLTFNKVIKP CMKKTIYENEREIKGYEYQL YVYASDKLFRADISEDYKTR GRKLLRFNGPV

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	596

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MONELLIN	1

Entities:

Entity 1, MONELLIN 91 residues - Formula weight is not available

1

GLY

GLU

TRP

GLU

ILE

GLU

ILE

GLY

PRO

2

PHE

TYR

GLN

ASN

LEU

GLY

LYS

PHE

ALA

VAL

3

ASP

GLU

ASN

LYS

ILE

GLY

GLN

TYR

GLY

4

ARG

LEU

THR

PHE

ASN

LYS

VAL

ILE

LYS

PRO

5

CYS

MET

LYS

THR

ILE

TYR

GLU

ASN

GLU

6

ARG

GLU

ILE

LYS

GLY

TYR

GLU

TYR

GLN

LEU

7

TYR

VAL

TYR

ALA

SER

ASP

LYS

LEU

PHE

ARG

8

ALA

ASP

ILE

SER

GLU

ASP

TYR

LYS

THR

ARG

9

GLY

ARG

LYS

LEU

ARG

PHE

ASN

GLY

PRO

10

VAL

Samples:

sample_1: MONELLIN 2 mM; photassium phosphate 50 mM; H20 90%; D20 10%

sample_2: MONELLIN, [U-15N], 2 mM; photassium phosphate 50 mM; H20 90%; D20 10%

sample_3: MONELLIN 2 mM; photassium phosphate 50 mM; D20 100%

sample_4: MONELLIN, [U-15N], 2 mM; photassium phosphate 50 mM; D20 100%

sample_cond_1: pH: 7.0; temperature: 298 K; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	sample_cond_1
3D 15N-separated NOESY	not available	not available	sample_cond_1
DQF-COSY	not available	not available	sample_cond_1
HNHA	not available	not available	sample_cond_1
HSQC(exchange)	not available	not available	sample_cond_1

Software:

No software information available

NMR spectrometers:

Bruker DRX 500 MHz