BMRB Entry 4623

Title:
Internal xylan binding domain from C. fimi Xyn10A, R262G mutant
Deposition date:
2000-07-14
Original release date:
2001-02-15
Authors:
Simpson, P.; Hefang, X.; Bolam, D.; Gilbert, H.; Williamson, M.
Citation:

Citation: Simpson, P.; Xie, H.; Bolam, D.; Gilbert, H.; Williamson, M.. "The Structural Basis for the Ligand Specificity of Family 2 Carbohydrate-binding Modules"  J. Biol. Chem. 275, 41137-41142 (2000).
PubMed: 10973978

Assembly members:

Assembly members:
XYLANASE D, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 1708   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Cellulomonas fimi

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts97
1H chemical shifts517

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Xyn10A1

Entities:

Entity 1, Xyn10A 87 residues - Formula weight is not available

1   THRGLYCYSSERVALTHRALATHRARGALA
2   GLUGLUTRPSERASPGLYPHEASNVALTHR
3   TYRSERVALSERGLYSERSERALATRPTHR
4   VALASNLEUALALEUASNGLYSERGLNTHR
5   ILEGLNALASERTRPASNALAASNVALTHR
6   GLYSERGLYSERTHRARGTHRVALTHRPRO
7   ASNGLYSERGLYASNTHRPHEGLYVALTHR
8   VALMETLYSASNGLYSERSERTHRTHRPRO
9   ALAALATHRCYSALAGLYSER

Samples:

sample_1: XYLANASE D, [U-15N], 1.5 mM

sample_cond_1: pH: 5.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
TOCSYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
NOESYsample_1not availablesample_cond_1
E.COSYsample_1not availablesample_cond_1
HSQCsample_1not availablesample_cond_1
NOESY-HMQCsample_1not availablesample_cond_1
TOCSY-HMQCsample_1not availablesample_cond_1
HNHAsample_1not availablesample_cond_1
HNHBsample_1not availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 750 MHz

Related Database Links:

BMRB 4241
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks