BMRB Entry 4254

Title:
1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Sassharomyces cerevisiae in Both Its Free and DNA Bound Forms and 1H Assignments of the Free DNA
Deposition date:
1998-10-27
Original release date:
1999-10-26
Authors:
McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew
Citation:

Citation: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew. "1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Saccharomyces cerevisiae in Both Its Free and DNA Bound Forms and 1H Assignments of the Fee DNA"  J. Biomol. NMR 13, 397-398 (1999).

Assembly members:

Assembly members:
MLU1-BOX binding protein, polymer, 135 residues, 15436.7 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET22B

Data sets:
Data typeCount
13C chemical shifts242
15N chemical shifts126
1H chemical shifts488

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mbp11

Entities:

Entity 1, Mbp1 135 residues - 15436.7 Da.

1   SERASNGLNILETYRSERALAARGTYRSER
2   GLYVALASPVALTYRGLUPHEILEHISSER
3   THRGLYSERILEMETLYSARGLYSLYSASP
4   ASPTRPVALASNALATHRHISILELEULYS
5   ALAALAASNPHEALALYSALALYSARGTHR
6   ARGILELEUGLULYSGLUVALLEULYSGLU
7   THRHISGLULYSVALGLNGLYGLYPHEGLY
8   LYSTYRGLNGLYTHRTRPVALPROLEUASN
9   ILEALALYSGLNLEUALAGLULYSPHESER
10   VALTYRASPGLNLEULYSPROLEUPHEASP
11   PHETHRGLNTHRASPGLYSERALASERPRO
12   PROPROALAPROLYSHISHISHISALASER
13   LYSVALASPLYSLEUALAALAALALEUGLU
14   HISHISHISHISHIS

Samples:

sample_one: MLU1-BOX binding protein, [U-15N], 0.8 – 1.3 mM

sample_two: MLU1-BOX binding protein, [U-15N;U-13C], 0.8 – 1.3 mM

sample_three: MLU1-BOX binding protein0.8 – 1.3 mM

sample_conditions_one: pH: 7.6; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

    Related Database Links:

    BMRB 4256
    PDB
    DBJ GAA22190
    EMBL CAA52271 CAA98618 CAY78452
    GB AAS64332 AAS64333 AAS64335 AAS64336 AAS64338
    REF NP_010227
    SP P39678
    TPG DAA11800
    AlphaFold P39678

    Download HSQC peak lists in one of the following formats:
    CSV: Backbone or all simulated peaks
    SPARKY: Backbone or all simulated peaks