BMRB Entry 36180

Title:
Solution Structure of Thioredoxin-Like Effector Protein (TRX3) from Edwardsiella tarda
Deposition date:
2018-04-16
Original release date:
2019-05-20
Authors:
Sayed, A.; Mok, Y.
Citation:

Citation: Sayed, Ahmed; Chakraborty, Smarajit; Leung, Ka Yin; Sugii, Shigeki; Mok, Yu Keung. "Trxlp, a thioredoxin-like effector from Edwardsiella piscicida inhibits cellular redox signaling and nuclear translocation of NF-kB"  Int. J. Biol. Macromol. 148, 89-8101 (2020).
PubMed: 31945434

Assembly members:

Assembly members:
Thioredoxin (H-type,TRX-H), polymer, 122 residues, 13694.305 Da.

Natural source:

Natural source:   Common Name: Edwardsiella tarda   Taxonomy ID: 498217   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Edwardsiella Edwardsiella tarda

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)

Data sets:
Data typeCount
13C chemical shifts188
15N chemical shifts95
1H chemical shifts347

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 122 residues - 13694.305 Da.

1   METHISHISHISHISHISHISSERSERGLY
2   LEUVALPROARGGLYSERGLNALAALASER
3   VALGLUPROTYRSERASPALAALAPHETHR
4   GLNALAGLNALASERGLYALAPROVALLEU
5   VALASPVALTYRALAASPTRPCYSPROVAL
6   CYSLYSARGGLNGLUARGGLULEUTHRPRO
7   LEUPHEALAGLNPROALAGLNARGASPLEU
8   ARGVALPHELYSVALASNPHEASPTHRGLN
9   LYSALAALALEUGLNGLNPHEARGVALSER
10   GLNGLNSERTHRLEUILELEUTYRARGASN
11   GLYGLNGLUVALARGARGSERILEGLYGLU
12   THRSERPROSERALALEUSERASPPHELEU
13   THRARG

Samples:

sample_1: TRX3, [U-13C; U-15N], 0.5 mM; phosphate 20 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D CC(CO)NHsample_1isotropicsample_conditions_1
3D HCC(CO)NHsample_1isotropicsample_conditions_1
3D NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks