BMRB Entry 36096

Title:
NMR solution structure of SMO1, Sumo homologue in Caenorhabditis elegans
Deposition date:
2017-06-07
Original release date:
2019-08-21
Authors:
Gowda, C.; Surana, P.; Das, R.
Citation:

Citation: Surana, Parag; Gowda, Chandrakala; Tripathi, Vasvi; Broday, Limor; Das, Ranabir. "Structural and functional analysis of SMO-1, the SUMO homolog in Caenorhabditis elegans"  PLoS One 12, e0186622-e0186622 (2017).
PubMed: 29045470

Assembly members:

Assembly members:
Small ubiquitin-related modifier, polymer, 97 residues, 11048.223 Da.

Natural source:

Natural source:   Common Name: C. elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts364
15N chemical shifts88
1H chemical shifts547

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 97 residues - 11048.223 Da.

1   METHISHISHISHISHISHISMETALAASP
2   ASPALAALAGLNALAGLYASPASNALAGLU
3   TYRILELYSILELYSVALVALGLYGLNASP
4   SERASNGLUVALHISPHEARGVALLYSTYR
5   GLYTHRSERMETALALYSLEULYSLYSSER
6   TYRALAASPARGTHRGLYVALALAVALASN
7   SERLEUARGPHELEUPHEASPGLYARGARG
8   ILEASNASPASPASPTHRPROLYSTHRLEU
9   GLUMETGLUASPASPASPVALILEGLUVAL
10   TYRGLNGLUGLNLEUGLYGLY

Samples:

sample_1: SMO1, Small Ubiquitin like modifier in C. elegans, [U-13C; U-15N], 620 uM; NaCl 150 mM; sodium phosphate 25 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AvanceIII 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks