BMRB Entry 30496

Title:
Heterogeneous-Backbone Mimics of a Designed Disulfide-Rich Protein: Aib turn
Deposition date:
2018-07-20
Original release date:
2018-11-12
Authors:
Cabalteja, C.; Mihalko, D.; Horne, W.
Citation:

Citation: Cabalteja, C.; Mihalko, D.; Horne, W.. "Heterogeneous-Backbone Foldamer Mimics of a Computationally Designed, Disulfide-Rich Miniprotein"  Chembiochem. 20, 103-110 (2019).
PubMed: 30326175

Assembly members:

Assembly members:
entity_1, polymer, 28 residues, 3260.814 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: NDKCKELKKRYXGCEVRCDX PRYEVHCX

Data sets:
Data typeCount
1H chemical shifts187

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 28 residues - 3260.814 Da.

1   ASNASPLYSCYSLYSGLULEULYSLYSARG
2   TYRAIBGLYCYSGLUVALARGCYSASPDPR
3   PROARGTYRGLUVALHISCYSNH2

Samples:

sample_1: DSS 0.2 mM; Designed peptide NC_HEE_D1: Aib turn mutant 0.5 mM

sample_conditions_1: ionic strength: 10 mM; pH: 4.0; pressure: 1 atm; temperature: 275 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYsample_1isotropicsample_conditions_1
2D COSYsample_1isotropicsample_conditions_1
2D NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

ARIA v2.3, Linge, O'Donoghue and Nilges - structure calculation

NMR spectrometers:

  • Bruker Avance 700 MHz