BMRB Entry 30490

Title:
Solution structure of Rbfox2 RRM mimetic peptide CPfox7
Deposition date:
2018-07-03
Original release date:
2019-05-07
Authors:
Sun, Y.-T.; Varani, G.
Citation:

Citation: Sun, Yi-Ting; Shortridge, Matthew; Varani, Gabriele. "A Small Cyclic beta-Hairpin Peptide Mimics the Rbfox2 RNA Recognition Motif and Binds to the Precursor miRNA 20b."  Chembiochem 20, 931-939 (2019).
PubMed: 30537200

Assembly members:

Assembly members:
entity_1, polymer, 16 residues, 2024.398 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 36630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSRRFRFXPKIIFNQR

Data sets:
Data typeCount
1H chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 16 residues - 2024.398 Da.

1   GLYSERARGARGPHEARGPHEDPRPROLYS
2   ILEILEPHEASNGLNARG

Samples:

sample_1: CPfox7 3.0 mg/mL; potassium phosphate 10 mM; potassium chloride 10 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - processing

NMR spectrometers:

  • Bruker AvanceIII 600 MHz