BMRB Entry 30371

Title:
NMR Structural and biophysical functional analysis of intracellular loop 5 of the NHE1 isoform of the Na+/H+ exchanger.
Deposition date:
2017-11-06
Original release date:
2018-11-06
Authors:
McKay, R.; Wong, K.; Towle, K.; Fliegel, L.
Citation:

Citation: Wong, Ka Yee; McKay, Ryan; Liu, Yongsheng; Towle, Kaitlyn; Elloumi, Yesmine; Li, Xiuju; Quan, Sicheng; Dutta, Debajyoti; Sykes, Brian; Fliegel, Larry. "Diverse residues of intracellular loop 5 of the Na+/H+ exchanger modulate proton sensing, expression, activity and targeting"  Biochim. Biophys. Acta Biomembr. 1861, 191-200 (2019).
PubMed: 30071192

Assembly members:

Assembly members:
entity_1, polymer, 13 residues, 1624.988 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GLTWFINKFRIVK

Data sets:
Data typeCount
1H chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 13 residues - 1624.988 Da.

1   GLYLEUTHRTRPPHEILEASNLYSPHEARG
2   ILEVALLYS

Samples:

sample_1: peptide 5 ± 0.5 mM; Na+ 440 mM

sample_conditions_1: ionic strength: 440 mM; pH: 6; pressure: 960 mbar; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1D Protonsample_1isotropicsample_conditions_1
2D NOESYsample_1isotropicsample_conditions_1
2D HSQCsample_1isotropicsample_conditions_1
2D TOCSYsample_1isotropicsample_conditions_1

Software:

VNMRJ v4.2, Agilent - collection

NMRPipe v8.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - peak picking

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation

NMR spectrometers:

  • Varian VNMRS 600 MHz