BMRB Entry 28137

Title:
Triple Resonance Assignments for the Rabies Phosphoprotein RavP (1-152)
Deposition date:
2020-07-30
Original release date:
2020-08-31
Authors:
Jespersen, Nathan; Leyrat, Cedric; Gerard, Francine; Bourhis, Jean-Marie; Blondel, Danielle; Jamin, Marc; Barbar, Elisar
Citation:

Citation: Jespersen, Nathan; Leyrat, Cedric; Gerard, Francine; Bourhis, Jean-Marie; Blondel, Danielle; Jamin, Marc; Barbar, Elisar. "The LC8-RavP ensemble Structure Evinces A Role for LC8 in Regulating Lyssavirus Polymerase Functionality"  J. Mol. Biol. 431, 4959-4977 (2019).
PubMed: 31634467

Assembly members:

Assembly members:
Rabies_Phosphoprotein_RavP, polymer, 158 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rabies lyssavirus   Taxonomy ID: 11292   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lyssavirus Rabies Virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24d+

Data sets:
Data typeCount
13C chemical shifts258
15N chemical shifts89
1H chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RavP1

Entities:

Entity 1, RavP 158 residues - Formula weight is not available

GAHM sequence at the N-terminus is non-native, leftover from cleavage. C-terminus has a non-endogenous GS sequence.

1   GLYALAHISMETMETSERLYSILEPHEVAL
2   ASNPROSERALAILEARGALAGLYLEUALA
3   ASPLEUGLUMETALAGLUGLUTHRVALASP
4   LEUILEASNARGASNILEGLUASPASNGLN
5   ALAHISLEUGLNGLYGLUPROILEGLUVAL
6   ASPASNLEUPROGLUASPMETGLYARGLEU
7   HISLEUASPGLYGLYLYSSERPROASNPRO
8   GLYGLUMETALALYSVALGLYGLUGLYLYS
9   TYRARGGLUASPPHEGLNMETASPGLUGLY
10   GLUASPPROSERLEULEUPHEGLNSERTYR
11   LEUGLUASNVALGLYVALGLNILEVALARG
12   GLNILEARGSERGLYGLUARGPHELEULYS
13   ILETRPSERGLNTHRVALGLUGLUILEILE
14   SERTYRVALALAVALASNPHEPROASNPRO
15   PROGLYLYSSERSERGLUASPLYSSERTHR
16   GLNTHRTHRGLYARGGLUGLYSER

Samples:

sample_1: Rabies Phosphoprotein (RavP), [U-99% 13C; U-99% 15N], 150 uM; sodium chloride 100 mM; MES 25 mM; sodium azide 1 mM; EDTA 5 mM; beta-mercaptoethanol 5 mM; DSS, [U-2H], 100 uM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks