BMRB Entry 28077

Title:
Backbone 1H, 13C, 15N chemical shift assignments for BRCT_S3R_V63A
Deposition date:
2020-02-09
Original release date:
2020-03-09
Authors:
Ampapathi, Ravi; Vaishnav, Jayanti; Alam, Faiyaz
Citation:

Citation: Ampapathi, Ravi; Vaishnav, Jayanti; Alam, Faiyaz. "'Backbone 1H, 13C, 15N chemical shift assignments of BRCT'"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
BRCA1_C-terminal_(BRCT)_domain, polymer, 116 residues, 12167.65 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21d

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts220
15N chemical shifts72
1H chemical shifts72

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BRCT1

Entities:

Entity 1, BRCT 116 residues - 12167.65 Da.

Residues 1-16, 104-110 are from the vector and residues 111-116 is a HIS-Tag

1   METALASERMETTHRGLYGLYGLNGLNMET
2   GLYARGGLYSERGLUPHEASPGLUARGVAL
3   PROARGTHRLEUALAGLYLEUTHRILEVAL
4   VALTHRGLYSERLEUTHRGLYPHESERARG
5   ASPASPALALYSGLUALAILEVALALAARG
6   GLYGLYLYSALAALAGLYSERVALSERLYS
7   LYSTHRASNTYRVALVALALAGLYASPSER
8   PROGLYSERLYSTYRASPLYSALAALAGLU
9   LEUGLYVALPROILELEUASPGLUASPGLY
10   PHEARGARGLEULEUALAASPGLYPROALA
11   SERARGTHRLYSLEUALAALAALALEUGLU
12   HISHISHISHISHISHIS

Samples:

sample_1: BRCA1 C-terminal (BRCT) domain, [U-99% 13C; U-99% 15N], 0.5 mM

sample_conditions_1: ionic strength: 200 mM; pH: 8.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

SPARKY v3.113, Goddard - chemical shift assignment

NMR spectrometers:

  • Agilent DD2 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks